Phospho Antibodies | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p100/p52 (Phospho-Ser869) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NFKB2-Ser869 antibody (836-885 aa) (STJ90342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using NF-kappaB p100 (Phospho-Ser872) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-NFKB2-Ser872 antibody (838-887 aa) (STJ90535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of HeLa cells, using Phospho-I Kappa B Alpha-S32 antibody (STJ116373) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-NFKBIA-S32 antibody (STJ116373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Phospho-I Kappa B Alpha-S32 rabbit monoclonal antibody (STJ11102564) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-Phospho-NFKBIA-S32 antibody [S4MR] (STJ11102564)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-I Kappa B Alpha-S32/36 Rabbit polyclonal antibody (STJ11105785) at 1:500 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-NFKBIA-S32/36 antibody (STJ11105785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Phospho-I Kappa B Alpha-S36 polyclonal antibody (STJ11101107) at 1:1000 dilution or IKB alpha antibody (A11397). HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-NFKBIA-S36 antibody (STJ11101107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Phospho-I Kappa B Alpha-S36 rabbit monoclonal antibody (STJ11102588) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight or treated by LPS (1 Mu g/mL) at 37 °C for 6 hours. Both NIH/3T3 cells and C6 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Phospho-NFKBIA-S36 antibody [S8MR] (STJ11102588)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-NFKBIA-Ser-32/Ser-36 antibody [39A1413] (STJA0005442)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NFKBIA-Ser32 antibody (STJ194713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of rat-spleen tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-NFKBIA-Ser32/S36 antibody (15-64 aa) (STJ90311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-NFKBIA-Tyr-305 antibody (STJA0005444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-NFKBIA-Tyr-42 antibody (STJA0005443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£365.50

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Immunohistochemistry analysis of paraffin-embedded human lymph node, using IkappaB-alpha (Phospho-Tyr305) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NFKBIA-Tyr305 antibody (268-317 aa) (STJ90912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NFKBIA-Tyr42 antibody (9-58 aa) (STJ90312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of HeLa cell lines, using Phospho-I Kappa B Alpha-Y42 antibody (STJ22317). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-NFKBIA-Y42 antibody (STJ22317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-I Kappa B-Beta (S23) Polyclonal Antibody

Anti-Phospho-NFKBIB-Ser23 antibody (8-57 aa) (STJ90313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-I Kappa B-Beta (T19) Polyclonal Antibody

Anti-Phospho-NFKBIB-Thr19 antibody (4-53 aa) (STJ90460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-NFKBIE-Ser22 antibody (131-180 aa) (STJ91018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-NGEF-Tyr-87 antibody (STJA0005409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-NIFK-Thr234 antibody (200-249 aa) (STJ91319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NKX2-1-Ser327 antibody (STJ194753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-NLRC4-Ser-533 antibody (STJA0005486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-NLRC4-Ser-533 antibody [M549] (STJA0005487)

Clonality :

Monoclonal

Host :

Rat

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-NMDA Epsilon 1/2-Tyr1246/1252 antibody (1216-1265 aa) (STJ91307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of HeLa cells, using Phospho-NMDAR1-S896/S897 antibody (STJ11103722) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 30 minutes and PMA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-NMDAR1-S896/S897 antibody (STJ11103722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-NMDAR1-S897 antibody (STJ11107680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using n-NOS (Phospho-Ser852) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NOS1-Ser852 antibody (818-867 aa) (STJ90355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-NOS2-Tyr-1055 antibody (STJA0005496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using iNOS (Phospho-Tyr151) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NOS2-Tyr151 antibody (117-166 aa) (STJ91227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of SKOV3 cells, using Phospho-eNOS-S1177 antibody (STJ110855). SKOV3 cells were treated by EGF. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-NOS3-S1177 antibody (STJ110855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of Jurkat cells, using Phospho-eNOS-S1177 antibody (STJ22320). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-NOS3-S1177 antibody (STJ22320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of 293T lysates, using Phospho-eNOS-S1177 antibody (STJ11105869) at 1:7000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37 °C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-NOS3-S1177 antibody [S5869RM] (STJ11105869)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Anti-Phospho-NOS3-Ser-1177 antibody (STJA0005493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-NOS3-Ser-632 antibody [M232] (STJA0005491)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western Blot analysis of 1 HEK-293 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NOS3-Ser114 antibody (STJA0010055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, NOS3 (phospho Ser1177) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-NOS3-Ser1177 antibody (1144-1193 aa) (STJ90674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of K562 cells, cell treated or untreated by EGF 20ng/ml 30'. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-Phospho-NOS3-Ser1179 antibody (STJ193218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells treated with EGF 40nM 30', using eNOS (Phospho-Ser615) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-NOS3-Ser615 antibody (581-630 aa) (STJ90541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of mouse heart, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NOS3-Ser633 antibody (STJA0009921)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-eNOS-T495 antibody (STJ110856) at 1:1000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-NOS3-T495 antibody (STJ110856)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of 293 cells using Phospho-NOS3 (T494) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-NOS3-Thr494 antibody (462-511 aa) (STJ90356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-NOS3-Tyr-657 antibody (STJA0005492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NPM1-Ser4 antibody (STJ194732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western Blot analysis of RAW 264. 7 cell, Raji cell, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NPM1-Ser70 antibody (STJA0010019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Phospho-NPM1-T199 polyclonal antibody (STJ11100977) at 1:1000 dilution or NPM1 antibody (A17983). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. PC-12 cells were treated by Nocodazole (1 µg/mL) at 37 °C for 16 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-NPM1-T199 antibody (STJ11100977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of B23 (phospho Thr199) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with forskolin 40nM 30', 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-NPM1-Thr199 antibody (171-220 aa) (STJ91062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-NPM1-Thr234 antibody (201-250 aa) (STJ90546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NPM1-Thr95 antibody (STJ194733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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