Rabbit Monoclonal Antibodies | St John's Laboratory

Anti-Phospho-Ezrin/Radixin/Moesin-Thr567/Thr564/Thr558 antibody [PT0916R] (STJA0040055)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-FAK-Tyr397 antibody [PT0915R] (STJA0039806)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-FAK-Tyr576/Tyr577 antibody [PT1269R] (STJA0040090)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-Flk-1/VEGFR2-Tyr1175 antibody [PT0865R] (STJA0039919)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

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Anti-Phospho-FOXO3-S253 antibody [S5793RM] (STJ11105793)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of Phospho-FOXO3A (Ser253) in 3T3 lysates using Phospho-FOXO3A (Ser253) antibody.

Anti-Phospho-FOXO3-Ser253 antibody (248-260 aa) [R06-2G6] (STJA0011330)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£246.50

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Anti-Phospho-FOXO3A-Ser253 antibody [PT0061R] (STJA0039645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-Glycogen synthase 1-Ser641 antibody [PT1289R] (STJA0040145)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-GSK3 Alpha/Beta-Tyr216/279 antibody [PT0733R] (STJA0039234)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-GSK3 Beta-Ser9 antibody [PT0078R] (STJA0039695)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of extracts of NIH/3T3, using Phospho-GSK3 Alpha-S21+GSK3 Beta-S9 antibody (STJ11105830) at 1:800 dilution. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-GSK3alpha + GSK3Beta-S21-S9 antibody [S5830RM] (STJ11105830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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Western blot analysis of various lysates using Phospho-GSK3 Beta-S9 Rabbit mAb (STJ11102623) at 1:1000 dilution incubated overnight at 4 °C. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-GSK3B-S9 antibody [S3MR] (STJ11102623)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of various lysates using Phospho-GYS1-S641 Rabbit mAb (STJ11105699) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-GYS1-S641 antibody [S6MR] (STJ11102626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of extracts of Jurkat cells, using Phospho-HDAC4-S632 antibody (STJ11105833) at1:2000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-HDAC4-S632 antibody [S5833RM] (STJ11105833)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-Phospho-HDAC5-Ser498 antibody [PT1439R] (STJA0040328)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-HER2-Tyr1221/1222 antibody [PT0740R] (STJA0039238)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA/CHIP/Cut&Tag

Reactivity :

Human

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Anti-Phospho-HER2/HER4-Tyr1284 antibody [PT1236R] (STJA0040156)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using Phospho-Histone H1. 3-T17/Histone H1. 4-T17 Rabbit mAb (STJ11102629) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H1.3/Histone H1.4-T17/T17 antibody [S9MR] (STJ11102629)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H2AX-S139 Rabbit mAb (STJ11102561) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H2A-S139 antibody [S1MR] (STJ11102561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-Histone H2A.X-Ser139 antibody [PT0330R] (STJA0039611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-Histone H3-Ser10/Thr11 antibody [PT0977R] (STJA0039792)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3. 3-T3 Rabbit mAb (STJ11102645) at 1:1000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-Histone H3-T3 antibody [S5MR] (STJ11102645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Anti-Phospho-Histone H3-Thr3 antibody [PT0291R] (STJA0039220)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA/CHIP/Cut&Tag

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from 293T cells, using Phospho-HSF1-S326 Rabbit mAb (STJ11102636) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-HSF1-S326 antibody [S6MR] (STJ11102636)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-Phospho-HSL-Ser853 antibody [PT1168R] (STJA0040219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-HSP27-Ser82 antibody [PT1395R] (STJA0040380)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£0.00

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S78 Rabbit mAb (STJ11102612) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-HSPB1-S78 antibody [S2MR] (STJ11102612)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (STJ11102611) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-HSPB1-S82 antibody [S1MR] (STJ11102611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Anti-Phospho-I Kappa B-Alpha-Ser32 antibody [PT0899R] (STJA0039844)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-I Kappa B-Alpha-Ser36 antibody [PT1061R] (STJA0039973)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-IGF1R/Insulin Receptor Beta-Tyr1135/1136&Tyr1150/1151 antibody [PT0867R] (STJA0039136)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-IKK-Alpha/Beta-Ser176/Ser180 antibody [PT0746R] (STJA0040295)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-Integrin Beta 2-Ser745 antibody [PT1301R] (STJA0040103)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from Karpas 299 cells using Phospho-IRAK4-Ser346 Rabbit monoclonal antibody (STJ11105898) at 1:1000 dilution. Karpas 299 cells were treated by IL-1 Beta (200 ng/ml) at 37 °C for 15 minutes after serum starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 75s.

Anti-Phospho-IRAK4-Ser346 antibody [S5898RM] (STJ11105898)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-Phospho-IRE1 Alpha-Ser724 antibody [PT1195R] (STJA0040223)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from HeLa cells, using Phospho-IRF3-S386 Rabbit mAb (STJ11102584) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-IRF3-S386 antibody [S4MR] (STJ11102584)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-Phospho-IRF3-Ser386 antibody [PT0984R] (STJA0039769)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

£0.00

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Anti-Phospho-IRF3-Ser396 antibody [PT0875R] (STJA0039777)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-JAK1-Tyr1034/1035 antibody [PT0991R] (STJA0040088)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-Phospho-JNK1 + JNK2 + JNK3-T183/Y185-T183/Y185-T221/Y223 antibody [S5826RM] (STJ11105826)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-JNK1/2/3-Thr183/183/221 antibody [PT0914R] (STJA0039805)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Jun-S63 Rabbit mAb (STJ11102553) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-JUN-S63 antibody [S3MR] (STJ11102553)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Jun-T91 Rabbit mAb (STJ11102592) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-JUN-T91 antibody [S2MR] (STJ11102592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-Jun/JunD-Ser73/100 antibody [PT0912R] (STJA0039803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of HeLa cells, using Phospho-JunD-S255 antibody (STJ11105821) at1:2000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-JUND-S255 antibody [S5821RM] (STJ11105821)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-JunD-S255 Rabbit mAb (STJ11102638) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-JUND-S255 antibody [S8MR] (STJ11102638)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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Western blot analysis of A-431 lysates, using Phospho-VEGF Receptor 2-Y1175 antibody (STJ11105860) at 1:3000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-KDR-Y1175 antibody [S5860RM] (STJ11105860)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-Phospho-LATS1-Thr1079 antibody [PT1358R] (STJA0040058)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£0.00

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