Rabbit Monoclonal Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using GSC Rabbit monoclonal antibody (STJ11103157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GSC antibody (1-100) [S7MR] (STJ11103157)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Anti-GSDMA antibody [SRM14551] (STJ11109281)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of lysates from HeLa cells, using GSDMC Rabbit monoclonal antibody (STJ11104233) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GSDMC antibody (1-150) [S4233RM] (STJ11104233)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from RAW 264. 7 cells using GSDMC Rabbit monoclonal antibody (STJ11105574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-GSDMC antibody (1-150) [S5574RM] (STJ11105574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit monoclonal antibody (STJ11105210) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Anti-GSDMD antibody (276-484) [S5210RM] (STJ11105210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit monoclonal antibody (STJ11103428) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-GSDMD antibody (346-484) [S8MR] (STJ11103428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of GSDMD in Jurkat lysates using GSDMD antibody.

Anti-GSDMD antibody (Full-length) [R06-5C5] (STJA0013051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£264.50

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Western blot analysis of DFNA5/GSDME in U251MG, U2OS, HepG2, Hela lysates using GSDME antibody.

Anti-GSDME antibody (1-270 aa) [R07-5I1] (STJA0011231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£246.50

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Anti-GSK-3 Alpha/Beta antibody [SRM14555] (STJ11109285)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-GSK-3 Alpha/Beta antibody [SRM14556] (STJ11109286)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of Rat lung, using GSK-3 Alpha/ Beta antibody (STJ11104691) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GSK-3-alpha/Beta antibody (1-100) [S4691RM] (STJ11104691)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Anti-GSK3 Alpha antibody [PT0615R] (STJA0039578)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-GSK3 Alpha/Beta antibody [PT0413R] (STJA0039525)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-GSK3 Beta antibody [PT0816R] (STJA0039156)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-GSK3 Beta antibody [SRM13034] (STJ11107764)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-GSK3 Beta antibody {ABflo® 647} (STJ11109020)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g GSK3 Alpha antibody (STJ11101598). Western blot was performed from the immunoprecipitate using GSK3 Alpha antibody (STJ11101598) at a dilution of 1:1000.

Anti-GSK3A antibody (1-100) [S8MR] (STJ11101598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using Gelsolin Rabbit monoclonal antibody (STJ11101830) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-GSN antibody (400-500) [S0MR] (STJ11101830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using GSR Rabbit monoclonal antibody (STJ11102104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GSR antibody (423-522) [S4MR] (STJ11102104)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of NIH-3T3 cells using Glutathione Synthetase (GSS) (GSS) Rabbit monoclonal antibody (STJ11101358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GSS antibody (350-450) [S8MR] (STJ11101358)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunoprecipitation analysis of 300 Mu g extracts of cells using 3 Mu g GST-Tag antibody (STJ11107585). Western blot was performed from the immunoprecipitate using GST-Tag antibody (STJ11107585) at a dilution of 1:5000.

Anti-GST-Tag antibody (1-218 aa) [SRM5514] (STJ11107585)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/ELISA

Reactivity :

Species Independent

£135.50 - £249.50

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Immunoprecipitation of GST-Tag from 300 µg extracts of 293F cells transfected with a MYOD1 expression vector containing a single N-terminal GST-Tag was performed using 20 µl of Magnetic Beads-conjugated Rabbit anti GST-Tag mAb (STJ11107597). Magnetic Beads-conjugated Rabbit IgG isotype control (STJ11107561) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti GST-Tag mAb (STJ11103710) at a dilution of 1:2000.

Anti-GST-Tag antibody (1-218 aa) [SRM5524] {Magnetic Beads} (STJ11107597)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP

Reactivity :

Species Independent

£135.50 - £249.50

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Western blot analysis of lysates from GST protein, using Rabbit anti GST-Tag mAb (STJ11103710) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-GST-Tag antibody (1-239) [S0MR] (STJ11103710)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Species Independent

£135.50 - £249.50

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Western blot analysis of lysates from GST fusion protein, using Rabbit anti GST-Tag mAb (STJ11105768) at 1:20000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 20ng per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-GST-Tag antibody (1-239) [S5768RM] (STJ11105768)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Species Independent

£135.50 - £249.50

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Anti-GST-Tag antibody [SRM] (STJ11107590)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP

Reactivity :

Species independent

£135.50 - £249.50

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Immunoprecipitation analysis of 300 Mu g extracts of cells using 3 Mu g Affinity Gel-conjugated Rabbit anti GST-Tag antibody (STJ11107588). Western blot was performed from the immunoprecipitate using Affinity Gel-conjugated Rabbit anti GST-Tag antibody (STJ11107588) at a dilition of 1:5000.

Anti-GST-Tag antibody [SRM] {Affinity Gel} (STJ11107588)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP

Reactivity :

Species independent

£135.50 - £249.50

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Western blot analysis of lysates from HepG2 cells, using GSTA4 Rabbit monoclonal antibody (STJ11105149) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GSTA4 antibody (1-100) [S5149RM] (STJ11105149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various lysates using GSTK1 Rabbit mAb (STJ11102523) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GSTK1 antibody (1-100) [S3MR] (STJ11102523)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using GSTO1 Rabbit monoclonal antibody (STJ11102121) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GSTO1 antibody (1-100) [S1MR] (STJ11102121)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using GST3/GSTP1 antibody (STJ11101599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-GSTP1 antibody (100-201) [S9MR] (STJ11101599)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates, using GSTT1 antibody (STJ11105007) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GSTT1 antibody (1-240 aa) [S5007RM] (STJ11105007)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of GTF2F2 in paraffin-embedded mouse testis tissue using GTF2F2 Rabbit monoclonal antibody (STJ11102690) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-GTF2F2 antibody (1-100) [S0MR] (STJ11102690)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunocytochemistry analysis of General Transcription Factor II I (green) in Hela using General Transcription Factor II I antibody, and DAPI (blue).

Anti-GTF2I antibody (66-80 aa) [R01-1F4] (STJA0012614)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£264.50

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Immunohistochemistry analysis of paraffin-embedded human colon using GTPBP4 Rabbit monoclonal antibody (STJ11102145) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GTPBP4 antibody (2-92) [S5MR] (STJ11102145)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded Human tonsil using GTPBP4/NOG1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-GTPBP4 antibody (52-65 aa) [R07-8G0] (STJA0012398)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£264.50

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Immunofluorescence analysis of NIH-3T3 cells using Guanylyl Cyclase beta 1 (GUCY1B3) (GUCY1B3) Rabbit monoclonal antibody (STJ11101926) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GUCY1B3 antibody (1-100) [S6MR] (STJ11101926)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunocytochemistry analysis of Glycophorin C (green) in K562 using Glycophorin C antibody, and DAPI (blue).

Anti-GYPC antibody (109-128 aa) [R04-8F4] (STJA0010780)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£264.50

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Western blot analysis of extracts of various cell lines, using Glycophorin C (GYPC) rabbit monoclonal antibody (STJ11101337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GYPC antibody (49-128) [S7MR] (STJ11101337)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates using Glycogen synthase 1 (Glycogen synthase 1 (GYS1) ) Rabbit mAb (STJ11102394) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-GYS1 antibody (638-737) [S4MR] (STJ11102394)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-GZMA antibody [SRM15026] (STJ11109756)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IF/ICC/ELISA

Reactivity :

Human

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Anti-H2-Ab1 antibody [SRM14761] (STJ11109491)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Mouse

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Western blot analysis of various lysates using macroH2A. 1 Rabbit mAb (STJ11102416) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-H2AFY antibody (200-300) [S6MR] (STJ11102416)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Formalin-fixed, paraffin-embedded human malignant giant cell tumor of bone stained with anti-H3. 3 G34W antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells.

Anti-H3.3G34W antibody [ZR459] (STJ180677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£179.50 - £714.50

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Immunofluorescence analysis of NIH/3T3 cells using H6PD/GDH Rabbit mAb (STJ11107303) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-H6PD/GDH antibody (541-791 aa) [SRM5393] (STJ11107303)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Anti-HA-Tag antibody [PT0523R] (STJA0039455)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

0

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Anti-HA-Tag antibody [SRM15110] (STJ11109840)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Species Independent

£0.00

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with SCRN1-HA-GFP (N terminal) using HRP-conjugated Rabbit anti HA-Tag mAb (STJ11107598) at1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 5s.

Anti-HA-Tag antibody [SRM5525] {HRP} (STJ11107598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Species Independent

£135.50 - £249.50

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Immunoprecipitation of LDHA-3X HA in 300 µg extracts from 293T cells transfected with LDHA-3X HA using 20 µl Magnetic Beads-conjugated Rabbit anti HA-Tag mAb (STJ11107599). Western blot analysis was performed using HRP-conjugated Mouse anti HA-Tag mAb (STJ11107576) at 1:5000 dilution.

Anti-HA-Tag antibody [SRM] {Magnetic Beads} (STJ11107599)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP

Reactivity :

Species independent

£135.50 - £249.50

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