Anti-Coenzyme NAD antibody [S4155RM] (STJ11104155)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Coenzyme NAD antibody for use in DB and ELISA in species independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | DB/ELISA |
| Host | Rabbit |
| Reactivity | Species Independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4155RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | DB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Specificity | NAD |
Additional Info
| Background | The coenzyme NAD is involved in oxidation–reduction reactions critical for glycolysis, fatty acid oxidation, the TCA cycle, and complex I of the mitochondrial respiratory chain and also is a key regulator of autophagy. At least two different mechanisms are involved. First, the NAD+-dependent deacetylase SIRT1 activates autophagy by directly deacetylating ATG proteins. Under starvation conditions, the increased NAD+/NADH ratio activates SIRT1, which results in stimulation of mitophagy. Second, the hydrogen of NADH can be transferred to NADP+ to form NADPH via the energy-linked transhydrogenase. In the fed state, when the NAD+/NADH ratio falls, NADPH inhibits autophagy by scavenging of ROS via the glutathione peroxidase-glutathione reductase system and by preventing the production of ROS at complex 1 of the respiratory chain. |
Information sourced from Uniprot.org