Rabbit Monoclonal Antibodies | St John's Laboratory

Western blot analysis of lysates from 293T cells, using Phospho-ErbB3/HER3-Y1222 Rabbit mAb (STJ11102622) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-ERBB3-Y1222 antibody [S2MR] (STJ11102622)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Western blot analysis of extracts from MCF7 cells, using Phospho-ErbB3/HER3-Y1289 Rabbit monoclonal antibody (STJ11105873) at 1:1000 dilution. MCF7 cells were treated by neuregulin at 37 °C for 15 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.

Anti-Phospho-ERBB3-Y1289 antibody [S5873RM] (STJ11105873)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Anti-Phospho-ErbB4/HER4-Y1284 antibody [SRM13706] (STJ11108436)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Anti-Phospho-Erk 1/2-Thr202/Tyr204 antibody [PT0643R] (STJA0039299)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Western blot analysis of lysates from Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (STJ11102556) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-ERK1/ERK2-T202/T185 antibody [S6MR] (STJ11102556)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ESR Alpha-S118 Rabbit mAb (STJ11102593) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-ESR1-S118 antibody [S3MR] (STJ11102593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€179.38 - €773.13

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Anti-Phospho-Ezrin/Radixin/Moesin-Thr567/Thr564/Thr558 antibody [PT0916R] (STJA0040055)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-FAK-Tyr397 antibody [PT0915R] (STJA0039806)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-FAK-Tyr576/Tyr577 antibody [PT1269R] (STJA0040090)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-FAK-Y576/Y577 antibody [SRM13711] (STJ11108441)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

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Anti-Phospho-Flk-1/VEGFR2-Tyr1175 antibody [PT0865R] (STJA0039919)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

€356.25 - €675.00

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Anti-Phospho-FOXO1 + FOXO3A+ FOXO4-T24+T32+T28 antibody [SRM13687] (STJ11108417)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of Phospho-FOXO3A (Ser253) in 3T3 lysates using Phospho-FOXO3A (Ser253) antibody.

Anti-Phospho-FOXO3-Ser253 antibody (248-260 aa) [R06-2G6] (STJA0011330)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€308.13

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Anti-Phospho-FOXO3A-Ser253 antibody [PT0061R] (STJA0039645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-GATA3-S308-S308 antibody [SRM15115] (STJ11109845)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

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Anti-Phospho-Glycogen synthase 1-Ser641 antibody [PT1289R] (STJA0040145)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-GSK3 Alpha/Beta-Tyr216/279 antibody [PT0733R] (STJA0039234)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-GSK3 Beta + GSK3 Alpha-Y216+Y279 antibody [SRM13708] (STJ11108438)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

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Anti-Phospho-GSK3 Beta-Ser9 antibody [PT0078R] (STJA0039695)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€187.50 - €675.00

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Western blot analysis of extracts of NIH/3T3, using Phospho-GSK3 Alpha-S21+GSK3 Beta-S9 antibody (STJ11105830) at 1:800 dilution. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-GSK3alpha + GSK3Beta-S21-S9 antibody [S5830RM] (STJ11105830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€148.13 - €651.88

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Western blot analysis of various lysates using Phospho-GSK3 Beta-S9 Rabbit mAb (STJ11102623) at 1:1000 dilution incubated overnight at 4 °C. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-GSK3B-S9 antibody [S3MR] (STJ11102623)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€148.13 - €651.88

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Western blot analysis of various lysates using Phospho-GYS1-S641 Rabbit mAb (STJ11105699) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-GYS1-S641 antibody [S6MR] (STJ11102626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€148.13 - €651.88

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Anti-Phospho-HDAC4+ HDAC5+ HDAC9-S246&S259&S220 antibody [SRM13688] (STJ11108418)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of Jurkat cells, using Phospho-HDAC4-S632 antibody (STJ11105833) at1:2000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-HDAC4-S632 antibody [S5833RM] (STJ11105833)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Anti-Phospho-HDAC5-Ser498 antibody [PT1439R] (STJA0040328)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-HER2-Tyr1221/1222 antibody [PT0740R] (STJA0039238)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA/CHIP/Cut&Tag

Reactivity :

Human

€187.50 - €675.00

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Anti-Phospho-HER2/HER4-Tyr1284 antibody [PT1236R] (STJA0040156)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Western blot analysis of various lysates using Phospho-Histone H1. 3-T17/Histone H1. 4-T17 Rabbit mAb (STJ11102629) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H1.3/Histone H1.4-T17/T17 antibody [S9MR] (STJ11102629)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H2AX-S139 Rabbit mAb (STJ11102561) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H2A-S139 antibody [S1MR] (STJ11102561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€179.38 - €773.13

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Anti-Phospho-Histone H2A.X-Ser139 antibody [PT0330R] (STJA0039611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-Histone H3-S10 antibody [SRM13680] (STJ11108410)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

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Anti-Phospho-Histone H3-S28 antibody [SRM13693] (STJ11108423)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat/Other

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Anti-Phospho-Histone H3-Ser10/Thr11 antibody [PT0977R] (STJA0039792)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-Histone H3-T11 antibody [SRM13698] (STJ11108428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ChIP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3. 3-T3 Rabbit mAb (STJ11102645) at 1:1000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-Histone H3-T3 antibody [S5MR] (STJ11102645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

€148.13 - €651.88

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Anti-Phospho-Histone H3-Thr3 antibody [PT0291R] (STJA0039220)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA/CHIP/Cut&Tag

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-Histone H3.3-S31 antibody [SRM13685] (STJ11108415)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

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Western blot analysis of lysates from 293T cells, using Phospho-HSF1-S326 Rabbit mAb (STJ11102636) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-HSF1-S326 antibody [S6MR] (STJ11102636)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€179.38 - €773.13

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Anti-Phospho-HSL-Ser853 antibody [PT1168R] (STJA0040219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-HSP27-Ser82 antibody [PT1395R] (STJA0040380)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€187.50 - €675.00

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Anti-Phospho-HSP27/HSPB1-S15 antibody [SRM13690] (STJ11108420)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S78 Rabbit mAb (STJ11102612) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-HSPB1-S78 antibody [S2MR] (STJ11102612)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€148.13 - €651.88

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (STJ11102611) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-HSPB1-S82 antibody [S1MR] (STJ11102611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€148.13 - €651.88

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Anti-Phospho-I Kappa B-Alpha-Ser32 antibody [PT0899R] (STJA0039844)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-I Kappa B-Alpha-Ser36 antibody [PT1061R] (STJA0039973)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-IGF1R/Insulin Receptor Beta-Tyr1135/1136&Tyr1150/1151 antibody [PT0867R] (STJA0039136)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€356.25 - €675.00

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Anti-Phospho-IKK-Alpha/Beta-Ser176/Ser180 antibody [PT0746R] (STJA0040295)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-Integrin Beta 2-Ser745 antibody [PT1301R] (STJA0040103)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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