• Western blot analysis of extracts of mouse brain, using GRIK2 rabbit monoclonal antibody (STJ11102533) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of 293T cells, using GRIK2 rabbit monoclonal antibody (STJ11102533) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of GRIK2 in paraffin-embedded human colon carcinoma tissue using GRIK2 rabbit monoclonal antibody (STJ11102533) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GRIK2 in paraffin-embedded human kidney tissue using GRIK2 rabbit monoclonal antibody (STJ11102533) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GRIK2 in paraffin-embedded mouse intestin tissue using GRIK2 rabbit monoclonal antibody (STJ11102533) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GRIK2 in paraffin-embedded rat kidney tissue using GRIK2 rabbit monoclonal antibody (STJ11102533) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-GRIK2 antibody (50-150) [S3MR] (STJ11102533)

SKU:
STJ11102533

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GRIK2 (50-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRIK2
Gene ID: 2898
Uniprot ID: GRIK2_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GRIK2 (Q13002).
Immunogen Sequence: MGAEELAFRFAVNTINRNRT LLPNTTLTYDTQKINLYDSF EASKKACDQLSLGVAAIFGP SHSSSANAVQSICNALGVPH IQTRWKHQVSDNKDSFYVSL Y
Tissue Specificity Expression is higher in cerebellum than in cerebral cortex.
Post Translational Modifications Sumoylation mediates kainate receptor-mediated endocytosis and regulates synaptic transmission. Sumoylation is enhanced by PIAS3 and desumoylated by SENP1. Ubiquitinated. Ubiquitination regulates the GRIK2 levels at the synapse by leading kainate receptor degradation through proteasome. Phosphorylated by PKC at Ser-868 upon agonist activation, this directly enhance sumoylation.
Function Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. Modulates cell surface expression of NETO2. Independent of its ionotropic glutamate receptor activity, acts as a thermoreceptor conferring sensitivity to cold temperatures. Functions in dorsal root ganglion neurons.
Protein Name Glutamate Receptor Ionotropic - Kainate 2
Gluk2
Excitatory Amino Acid Receptor 4
Eaa4
Glutamate Receptor 6
Glur-6
Glur6
Database Links Reactome: R-HSA-451307
Reactome: R-HSA-451308
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Alternative Antibody Names Anti-Glutamate Receptor Ionotropic - Kainate 2 antibody
Anti-Gluk2 antibody
Anti-Excitatory Amino Acid Receptor 4 antibody
Anti-Eaa4 antibody
Anti-Glutamate Receptor 6 antibody
Anti-Glur-6 antibody
Anti-Glur6 antibody
Anti-GRIK2 antibody
Anti-GLUR6 antibody

Information sourced from Uniprot.org

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