Anti-GSDMA antibody [SRM14551] (STJ11109281)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal GSDMA antibody for use in WB, IF-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14551 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:2000IF-P, 1:50-1:200ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GSDMA |
| Gene ID | 284110 |
| Uniprot ID | GSDMA_HUMAN |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Expressed predominantly in the gastrointestinal tract and, at a lower level, in the skin. Also detected in mammary gland. In the gastrointestinal tract, mainly expressed in differentiated cells, including the differentiated cell layer of esophagus and mucus-secreting pit cells of the gastric epithelium. Down-regulated in gastric cancer cells. |
| Post Translational Modifications | Cleavage by S.pyogenes SpeB relieves autoinhibition by releasing the N-terminal moiety (Gasdermin-A, N-terminal) that initiates pyroptosis. Palmitoylated. |
| Function | Gasdermin-A: This form constitutes the precursor of the pore-forming protein and acts as a sensor of infection: upon infection by S.pyogenes, specifically cleaved by S.pyogenes effector protein SpeB in epithelial cells, releasing the N-terminal moiety (Gasdermin-A, N-terminal) that binds to membranes and forms pores, triggering pyroptosis. Gasdermin-A, N-terminal: Pore-forming protein that causes membrane permeabilization and pyroptosis. Released upon cleavage by S.pyogenes effector protein SpeB, and binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. Pyroptosis triggers the elimination of the infected skin cell, depriving the pathogen of its protective niche, while inducing an inflammatory response. This ultimately prevents bacterial penetration of the epithelial barrier and a subsequent systemic dissemination of the pathogen. Binds to cardiolipin and other acidic phospholipids, such as phosphatidylserine, which mediate its targeting to the inner leaflet membrane. |
| Protein Name | Gasdermin-AGasdermin-1 Cleaved Into - Gasdermin-A - N-TerminalGsdma-Nt - Gasdermin-A - C-TerminalGsdma-Ct |
| Database Links | |
| Cellular Localisation | Gasdermin-A: CytoplasmPerinuclear RegionCytoplasmCytosolGasdermin-AN-Terminal: Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Gasdermin-A antibodyAnti-Gasdermin-1 Cleaved Into - Gasdermin-A - N-Terminal antibodyAnti-Gsdma-Nt - Gasdermin-A - C-Terminal antibodyAnti-Gsdma-Ct antibodyAnti-GSDMA antibodyAnti-GSDM antibodyAnti-GSDM1 antibodyAnti-FKSG9 antibody |
Information sourced from Uniprot.org