Western Blot Antibodies | St John's Laboratory

Anti-ARF1 antibody (STJ500153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

£438.50

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Anti-ARF1 antibody (STJA0006869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ARF1 antibody {Biotin} (STJ500154)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-ARF1 antibody {FITC} (STJ500155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using ARF12 Rabbit polyclonal antibody (STJ11104022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ARF12 antibody (710-818 aa) (STJ11104022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

O.sativa

£143.50 - £618.50

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Anti-ARF3 antibody (66-116) (STJA0006333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using ARF4 Rabbit polyclonal antibody (STJ117836) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARF4 antibody (1-180) (STJ117836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ72838 (0. 5µg/ml) staining of NIH3T3 (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ARF4 antibody (137-150) (STJ72838)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARF4 antibody (71-120 aa) (STJ91673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-ARF4 antibody (STJ500156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-ARF4 antibody {Biotin} (STJ500157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-ARF4 antibody {FITC} (STJ500158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis of lysates from wild type (WT) and ARF5 knockout (KO) 293T cells, using [KO Validated] ARF5 Rabbit polyclonal antibody (STJ11100862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ARF5 antibody (1-180) (STJ11100862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using ARF5 antibody (STJ22668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ARF5 antibody (1-180) (STJ22668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARF5 antibody (40-120 aa) (STJ190524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Confocal imaging of NIH/3T3 cells using ARF5 Rabbit monoclonal antibody (STJ11101858, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-ARF5 antibody (81-180) [S8MR] (STJ11101858)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of HeLa cells using ARF6 antibody (STJ22670) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ARF6 antibody (1-175) (STJ22670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARF6 antibody (30-110 aa) (STJ91674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of C6 cells using ARF6 Rabbit monoclonal antibody (STJ11101343) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ARF6 antibody (76-175) [S3MR] (STJ11101343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (STJ29198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ARFGAP1 antibody (1-220) (STJ29198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARFGAP1 antibody (171-220 aa) (STJ91671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Cow

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using ARFGAP2 Rabbit polyclonal antibody (STJ119821) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARFGAP2 antibody (242-521) (STJ119821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ARFGAP2 antibody (STJA0009100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ARFGAP3 antibody (280-360 aa) (STJ91672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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STJ70078 staining (2µg/ml) of mouse brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-ARFGAP3 antibody (C-Term) (STJ70078)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis of various lysates using ARFGEF1 Rabbit polyclonal antibody (STJ118910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ARFGEF1 antibody (220-420) (STJ118910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various cells using BIG2 Polyclonal Antibody

Anti-ARFGEF2 antibody (1491-1540 aa) (STJ91854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human thymus gland. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARFIP1 antibody (271-320 aa) (STJ91675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARFIP2 antibody (137-187 aa) (STJ194394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using ARFRP1 Rabbit polyclonal antibody (STJ116523) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARFRP1 antibody (1-201) (STJ116523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARFRP1 antibody (110-160 aa) (STJ195125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ARFRP1/ARP antibody [SRM13096] (STJ11107826)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of paraffin-embedded human liver cancer using Arginase 1 (ARG1) Rabbit polyclonal antibody (STJ26182) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARG1 antibody (1-322) (STJ26182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Whole cell lysates of HepG2 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Arginase-1 antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Predicted band size: 35 kDa

Anti-ARG1 antibody (200-322 aa) [ABT-Arg1] (STJ197017)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£180.00 - £261.00

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Western blot analysis of 293 cells using Arginase I Polyclonal Antibody

Anti-ARG1 antibody (61-110 aa) (STJ91676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescent image of mouse retina specifically labeling arginase (146-ARG, 1:500, green) and CD206 (red). The blue nuclear stain is DAPI. The image was kindly provided by Sarah Gough, University of Miami Miller School of Medicine.

Anti-ARG1-ARG1 antibody (STJA0003835)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC

Reactivity :

Guinea Pig/Human/Mouse/Rat

£488.50

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Western blot analysis of extracts of Rat kidney, using Arginase 2 (ARG2) antibody (STJ28438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ARG2 antibody (270-354) (STJ28438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Arginase II Polyclonal Antibody

Anti-ARG2 antibody (305-354 aa) (STJ91677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Arg3.1 antibody (C-Term) (STJ13100597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-Arg3.1 antibody (STJ13100002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-Arg3.1 antibody (STJ13100526)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-Arg3.1 antibody (STJ13100537)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-Arg3.1 antibody (STJ13100565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-Arginase 1 (ARG1) antibody [SRM15100] (STJ11109830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemistry analysis of paraffin-embedded human prostate cancer using Arginase 2 (Arginase 2 (ARG2) ) Rabbit monoclonal antibody (STJ11102965) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Arginase 2 antibody (255-354) [S5MR] (STJ11102965)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Arginase 2/ARG2 antibody (23-354aa) [AT7F8] (STJA0041764)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Anti-Arginase 2/ARG2 antibody (23-354aa) [AT7F8] (STJA0041912)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

£238.50 - £361.50

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STJ70719 (0. 01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arginase I antibody (C-Term) (STJ70719)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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