• Immunohistochemistry analysis of paraffin-embedded mouse brain using APOA1 antibody (STJ116143) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using APOA1 antibody (STJ116143) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using APOA1 antibody (STJ116143) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using APOA1 antibody (STJ116143) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-APOA1 antibody (25-267) (STJ116143)

SKU:
STJ116143

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOA1 (25-267) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOA1
Gene ID: 335
Uniprot ID: APOA1_HUMAN
Immunogen Region: 25-267
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human APOA1 (NP_000030.1).
Immunogen Sequence: DEPPQSPWDRVKDLATVYVD VLKDSGRDYVSQFEGSALGK QLNLKLLDNWDSVTSTFSKL REQLGPVTQEFWDNLEKETE GLRQEMSKDLEEVKAKVQPY LDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEK LSPLGEEMRDRARAHVDALR THLAPYSDELRQRLAARLEA LKENGGARLAEYHAKATEHL STLSEKAKPALEDLRQGLLP VLESFKVSFLSALEEYTKK
Tissue Specificity Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
Post Translational Modifications Glycosylated. Palmitoylated. Phosphorylation sites are present in the extracellular medium.
Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Protein Name Apolipoprotein A-I
Apo-Ai
Apoa-I
Apolipoprotein A1 Cleaved Into - Proapolipoprotein A-I
Proapoa-I - Truncated Apolipoprotein A-I
Apolipoprotein A-I(1-242
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1369062
Reactome: R-HSA-1989781
Reactome: R-HSA-2168880
Reactome: R-HSA-3000471
Reactome: R-HSA-3000480
Reactome: R-HSA-381426
Reactome: R-HSA-5682113
Reactome: R-HSA-8957275
Reactome: R-HSA-8963888
Reactome: R-HSA-8963896
Reactome: R-HSA-8963901
Reactome: R-HSA-8964011
Reactome: R-HSA-8964058
Reactome: R-HSA-9707616
Reactome: R-HSA-975634
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative Antibody Names Anti-Apolipoprotein A-I antibody
Anti-Apo-Ai antibody
Anti-Apoa-I antibody
Anti-Apolipoprotein A1 Cleaved Into - Proapolipoprotein A-I antibody
Anti-Proapoa-I - Truncated Apolipoprotein A-I antibody
Anti-Apolipoprotein A-I(1-242 antibody
Anti-APOA1 antibody

Information sourced from Uniprot.org

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