Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of various lysates using phospho-YAP1-S397 Rabbit pAb (STJ11101056) at 1:1000 dilution. NIH3T3 cells and C6 cells were treated by Sorbitol (0. 2M) at 37 °C for 60 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1s.

Anti-Phospho-YAP1-S397 antibody (STJ11101056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Immunofluorescence analysis of Hela cell. 1, YAP (phospho Ser127) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-YAP1-Ser127 antibody (93-142 aa) (STJ90868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Immunofluorescence analysis of Hela cell. 1, 14-3-3 Theta/Tau (phospho Ser232) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-YWHAQ-Ser232 antibody (196-245 aa) (STJ91063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from HeLa cells, using Phospho-YWHAZ-S58 Rabbit pAb (STJ22447). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-YWHAZ-S58 antibody (STJ22447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of various cells using Phospho-14-3-3 Zeta (S58) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-YWHAZ-Ser58 antibody (24-73 aa) (STJ91013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from Jurkat cells treated with UV 15', using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-YWHAZ-Thr232 antibody (196-245 aa) (STJ90869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-Zap-70/Syk-Tyr319/Tyr352 antibody [PT0710R] (STJA0039240)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

€342.00 - €648.00

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Western blot analysis of lysates from Jurkat cells treated with Ca+ 40nM 30', using ZAP-70 (Phospho-Tyr315) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ZAP70-Tyr315 antibody (281-330 aa) (STJ90842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr319) Antibody. The lane on the right is STJ90436

Anti-Phospho-ZAP70-Tyr319 antibody (286-335 aa) (STJ90436)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr493) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ZAP70-Tyr493 antibody (460-509 aa) (STJ90437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Confocal imaging of EL4 cells (treated with H‚‚O‚‚ and pervanadate) and EL4 cells (untreated) using Phospho-Zap70-Y319+Syk-Y352 Rabbit pAb (STJ11107734, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-Phospho-Zap70-Y319 + Y352 antibody (STJ11107734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse

€151.80 - €742.20

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Western blot analysis of lysates from Jurkat cells, using Phospho-ZAP70-Y493 Rabbit pAb (STJ22449) at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes after serum-starvation overnight or treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-ZAP70-Y493 antibody (STJ22449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Immunofluorescence analysis of Jurkat treated with H2O2 and Jurkat using Phospho-ZAP70-Y493 Rabbit monoclonal antibody (STJ11105888) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Phospho-ZAP70-Y493 antibody [S5888RM] (STJ11105888)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

€142.20 - €625.80

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Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using NIPA (Phospho-Ser354) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ZC3HC1-Ser354 antibody (320-369 aa) (STJ90545)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Western blot analysis of lysate from Jurkat cells, using phospho-ZFP598 (Phospho-Tyr306) antibody. STJ90438

Anti-Phospho-ZNF598-Tyr306 antibody (STJ90438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Anti-Phospholamban antibody [SRM14672] (STJ11109402)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Mouse/Rat

€142.20 - €625.80

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PLAP antibody western blot MCF-7 lysates STJA0037364

Anti-Phospholipase A2 Activator Protein antibody [R01-1N8] (STJA0037364)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

€160.20 - €337.80

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Phospho-lipase C gamma 1 antibody immunocytochemistry HeLa STJA0035321

Anti-Phospholipase C gamma 1 antibody [R01-6B4] (STJA0035321)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

€160.20 - €337.80

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ICC IF of phosphoserine phosphatase in A431 cells stained with STJA0041499 antibody

Anti-Phosphoserine phosphatase/PSPH antibody (1-225aa) [3G12] (STJA0041499)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

€263.40 - €399.00

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Western blot analysis of lysates from K562 and HepG2 cells, using AGXT2L2 Antibody. The lane on the right is blocked STJ91509

Anti-PHYKPL antibody (341-390 aa) (STJ91509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of L929 cells using PIGM Rabbit polyclonal antibody (STJ119837) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PIGM antibody (35-96) (STJ119837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PIK3R5 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PIK3R5 antibody (695-744 aa) (STJ95073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of 3T3 cells using PLA1A Polyclonal Antibody

Anti-PLA1A antibody (381-430 aa) (STJ95148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of THP-1, H460, A549, HT29, HeLa, mouse kidney, mouse colon cells using cPLA2 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PLA2G4A antibody (31-80 aa) (STJ96541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of Hela cell. 1, cPLA2 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PLA2G4A antibody (471-520 aa) (STJ92449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using PLA2G4D Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PLA2G4D antibody (531-580 aa) (STJ92451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PLA2G4E Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PLA2G4E antibody (401-450 aa) (STJ92452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Phospho-lipase A2 Activator Protein antibody immunocytochemistry HeLa cells STJA0012681

Anti-PLAA antibody (15-35 aa) [R06-1D7] (STJA0012681)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

€160.20 - €337.80

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Western blot analysis of various cells using PLC Beta 3 Polyclonal Antibody

Anti-PLCB3 antibody (503-552 aa) (STJ95155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-PLCE1 antibody (STJ191893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of various cells using PLC Gamma 1 Polyclonal Antibody diluted at 1：2000

Anti-PLCG1 antibody (736-785 aa) (STJ95157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PLCG2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PLCG2 antibody (1186-1235 aa) (STJ95159)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of A549 cells using PLCXD2 antibody (STJ116099).

Anti-PLCXD2 antibody (1-305) (STJ116099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PLD1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PLD1 antibody (527-576 aa) (STJ94983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of AD293 cells using NTE Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PNPLA6 antibody (1031-1080 aa) (STJ96728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using PPA2 Rabbit polyclonal antibody (STJ118264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PPA2 antibody (33-100) (STJ118264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of various lysates, using PGC1 Alpha rabbit polyclonal antibody (STJ114227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PPARGC1A antibody (610-798) (STJ114227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of extracts of various cell lines, using PGC1 Alpha antibody (STJ118021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PPARGC1A antibody (610-798) (STJ118021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Anti-PPARGC1A antibody (Internal) (STJ73782)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC/FC

Reactivity :

Human/Mouse/Rat

€375.00

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Immunofluorescence analysis of PC-12 cells using AMPK Alpha 1 Rabbit polyclonal antibody (STJ25112) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PRKAA1 antibody (1-300) (STJ25112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Immunofluorescence analysis of rat-lung tissue. 1, AMPK Alpha 1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PRKAA1 antibody (451-500 aa) (STJ91580)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis using AMPK Alpha 1 monoclonal antibody against Jurkat (1) , HeLa (2) , HepG2 (3) , MCF-7 (4) , Cos7 (5) , NIH/3T3 (6) , K562 (7) , HEK293 (8) , and PC-12 (9) cell lysate.

Anti-PRKAA1 antibody [2B7] (STJ97826)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€178.80 - €432.00

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Western blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) PC12 with AMPK a1 Mouse mAb diluted at 1:2, 000.

Anti-PRKAA1 antibody [5G11] (STJ97538)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunofluorescence analysis of NIH/3T3 cells using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PRKAA1/PRKAA2 antibody (13-559) (STJ114591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Immunofluorescence analysis of U2OS cells using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ119425) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKAA1/PRKAA2 antibody (5-280) (STJ119425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Immunofluorescence analysis of PC-12 cells using AMPK Alpha 2 Rabbit polyclonal antibody (STJ11103813) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKAA2 antibody (270-343) (STJ11103813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

€132.60 - €606.60

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Western blot analysis of various lysates, using PRKAA2 antibody (STJ115987) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKAA2 antibody (270-552) (STJ115987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of lysates from wild type (WT) and AMPK Alpha 2 knockout (KO) HCT116 cells, using [KO Validated] AMPK Alpha 2 Rabbit polyclonal antibody (STJ29478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PRKAA2 antibody (270-552) (STJ29478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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