Anti-BBS7 antibody (1-270) (STJ119759)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BBS7 (1-270) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BBS7 |
| Gene ID | 55212 |
| Uniprot ID | BBS7_HUMAN |
| Immunogen Region | 1-270 |
| Immunogen Sequence | MDLILNRMDYLQVGVTSQKT MKLIPASRHRATQKVVIGDH DGVVMCFGMKKGEAAAVFKT LPGPKIARLELGGVINTPQE KIFIAAASEIRGFTKRGKQF LSFETNLTESIKAMHISGSD LFLSASYIYNHYCDCKDQHY YLSGDKINDVICLPVERLSR ITPVLACQDRVLRVLQGSDV MYAVEVPGPPTVLALHNGNG GDSGEDLLFGTSDGKLALIQ ITTSKPVRKWEIQNEKKRG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human BBS7 (NP_060660.2). |
Additional Info
| Tissue Specificity | Isoform 2 is ubiquitously expressed. Isoform 1 is expressed in retina, lung, liver, testis, ovary, prostate, small intestine, liver, brain, heart and pancreas. |
| Function | The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. |
| Protein Name | Bbsome Complex Member Bbs7Bbs2-Like Protein 1Bardet-Biedl Syndrome 7 Protein |
| Database Links | Reactome: R-HSA-5620922 |
| Cellular Localisation | Cell ProjectionCilium MembraneCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentriolar SatelliteCilium Basal Body |
| Alternative Antibody Names | Anti-Bbsome Complex Member Bbs7 antibodyAnti-Bbs2-Like Protein 1 antibodyAnti-Bardet-Biedl Syndrome 7 Protein antibodyAnti-BBS7 antibodyAnti-BBS2L1 antibody |
Information sourced from Uniprot.org