• Immunohistochemistry analysis of paraffin-embedded human brain tissue, using BCAR3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from HeLa and Jurkat cells, using BCAR3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-BCAR3 antibody (761-810 aa) (STJ91828)

SKU:
STJ91828

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Breast cancer anti-estrogen resistance protein 3 (761-810 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCAR3
Gene ID: 8412
Uniprot ID: BCAR3_HUMAN
Immunogen Region: 761-810 aa
Specificity: BCAR3 Polyclonal Antibody detects endogenous levels of BCAR3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BCAR3 at the amino acid range 761-810
Post Translational Modifications Phosphorylated on tyrosine residues.
Function Acts as an adapter protein downstream of several growth factor receptors to promote cell proliferation, migration, and redistribution of actin fibers. Specifically involved in INS/insulin signaling pathway by mediating MAPK1/ERK2-MAPK3/ERK1 activation and DNA synthesis. Promotes insulin-mediated membrane ruffling. In response to vasoconstrictor peptide EDN1, involved in the activation of RAP1 downstream of PTK2B via interaction with phosphorylated BCAR1. Inhibits cell migration and invasion via regulation of TGFB-mediated matrix digestion, actin filament rearrangement, and inhibition of invadopodia activity. May inhibit TGFB-SMAD signaling, via facilitating BCAR1 and SMAD2 and/or SMAD3 interaction. Regulates EGF-induced DNA synthesis. Required for the maintenance of ocular lens morphology and structural integrity, potentially via regulation of focal adhesion complex signaling. Acts upstream of PTPRA to regulate the localization of BCAR1 and PTPRA to focal adhesions, via regulation of SRC-mediated phosphorylation of PTPRA. Positively regulates integrin-induced tyrosine phosphorylation of BCAR1. Acts as a guanine nucleotide exchange factor (GEF) for small GTPases RALA, RAP1A and RRAS. However, in a contrasting study, lacks GEF activity towards RAP1.
Protein Name Breast Cancer Anti-Estrogen Resistance Protein 3
Novel Sh2-Containing Protein 2
Sh2 Domain-Containing Protein 3b
Cellular Localisation Cytoplasm
Cell Junction
Focal Adhesion
Localization To Focal Adhesions Depends On Interaction With Ptpra
Alternative Antibody Names Anti-Breast Cancer Anti-Estrogen Resistance Protein 3 antibody
Anti-Novel Sh2-Containing Protein 2 antibody
Anti-Sh2 Domain-Containing Protein 3b antibody
Anti-BCAR3 antibody
Anti-NSP2 antibody
Anti-SH2D3B antibody
Anti-UNQ271 antibody
Anti-PRO308 antibody

Information sourced from Uniprot.org

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