• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g BAP31 antibody (STJ29136). Western blot was performed from the immunoprecipitate using BAP31 antibody (STJ29136) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using BAP31 Rabbit polyclonal antibody (STJ29136). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using BAP31 Rabbit polyclonal antibody (STJ29136) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BCAP31 antibody (127-246) (STJ29136)

SKU:
STJ29136

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BCAP31 (127-246) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCAP31
Gene ID: 10134
Uniprot ID: BAP31_HUMAN
Immunogen Region: 127-246
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 127-246 of human BAP31 (NP_001243376.1).
Immunogen Sequence: ATLLASNEAFKKQAESASEA AKKYMEENDQLKKGAAVDGG KLDVGNAEVKLEEENRSLKA DLQKLKDELASTKQKLEKAE NQVLAMRKQSEGLTKEYDRL LEEHAKLQAAVDGPMDKKEE
Tissue Specificity Ubiquitous. Highly expressed in neurons and discrete endocrine cells.
Post Translational Modifications Cleaved by CASP8 and other caspases.
Function Functions as a chaperone protein. Is one of the most abundant endoplasmic reticulum (ER) proteins. Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). Also serves as a cargo receptor for the export of transmembrane proteins. Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by stimulating the translocation of NDUFS4 and NDUFB11 from the cytosol to the mitochondria via interaction with TOMM40. In response to ER stress, delocalizes from the ER-mitochondria contact sites and binds BCL2. May be involved in CASP8-mediated apoptosis.
Protein Name B-Cell Receptor-Associated Protein 31
Bcr-Associated Protein 31
Bap31
6c6-Ag Tumor-Associated Antigen
Protein Cdm
P28
Database Links Reactome: R-HSA-111465
Reactome: R-HSA-75153
Reactome: R-HSA-8980692
Reactome: R-HSA-983170
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
May Shuttle Between The Er And The Intermediate Compartment/Cis-Golgi Complex
Associates With The Mitochondria-Associated Endoplasmic Reticulum Membrane Via Interaction With Tomm40
(Microbial Infection) Upon Sv40 Infection
Colocalizes With Dnajc18
Dnajb12 And Dnajb14 In Punctate Structures Within The Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-B-Cell Receptor-Associated Protein 31 antibody
Anti-Bcr-Associated Protein 31 antibody
Anti-Bap31 antibody
Anti-6c6-Ag Tumor-Associated Antigen antibody
Anti-Protein Cdm antibody
Anti-P28 antibody
Anti-BCAP31 antibody
Anti-BAP31 antibody
Anti-DXS1357E antibody

Information sourced from Uniprot.org

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