• Western blot analysis of extracts of 22Rv1 cells, using BCKDK antibody (STJ110483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using BCKDK antibody (STJ110483) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using BCKDK antibody (STJ110483) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of 22Rv1 cells, using BCKDK antibody (STJ110483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BCKDK antibody (153-412) (STJ110483)

SKU:
STJ110483

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BCKDK (153-412) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCKDK
Gene ID: 10295
Uniprot ID: BCKD_HUMAN
Immunogen Region: 153-412
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 153-412 of human BCKDK (NP_005872.2).
Immunogen Sequence: QLVRQLLDDHKDVVTLLAEG LRESRKHIEDEKLVRYFLDK TLTSRLGIRMLATHHLALHE DKPDFVGIICTRLSPKKIIE KWVDFARRLCEHKYGNAPRV RINGHVAARFPFIPMPLDYI LPELLKNAMRATMESHLDTP YNVPDVVITIANNDVDLIIR ISDRGGGIAHKDLDRVMDYH FTTAEASTQDPRISPLFGHL DMHSGAQSGPMHGFGFGLPT SRAYAEYLGGSLQLQSLQG
Tissue Specificity Ubiquitous.
Post Translational Modifications Autophosphorylated.
Function Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
Protein Name 3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial
Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase
Bckd-Kinase
Bckdhkin
Database Links Reactome: R-HSA-70895
Cellular Localisation Mitochondrion Matrix
Mitochondrion
Alternative Antibody Names Anti-3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial antibody
Anti-Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase antibody
Anti-Bckd-Kinase antibody
Anti-Bckdhkin antibody
Anti-BCKDK antibody

Information sourced from Uniprot.org

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