Immunofluorescence Antibodies | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PAK1/2/3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PAK Alpha/Beta/Gamma antibody (111-160 aa) (STJ94944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 3T3 cells using PAK Alpha/Beta/Gamma Polyclonal Antibody

Anti-PAK Alpha/Beta/Gamma antibody (391-440 aa) (STJ94943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH-3T3 cells using PAK1 Rabbit monoclonal antibody (STJ11101728). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PAK1 antibody (1-100) [S8MR] (STJ11101728)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using PAK Alpha Polyclonal Antibody diluted at 1：500

Anti-PAK1 antibody (178-227 aa) (STJ94941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and PAK2 knockout (KO) HeLa cells, using [KO Validated] PAK2 Rabbit polyclonal antibody (STJ11100066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PAK2 antibody (1-380) (STJ11100066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of 3T3 cells using PAK Gamma Polyclonal Antibody

Anti-PAK2 antibody (5-54 aa) (STJ94946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using PAK Gamma monoclonal antibody against HeLa (1) , Jurkat (2) , A549 (3) , HEK293 (4) and K562 (5) cell lysate.

Anti-PAK2 antibody [3B5] (STJ98304)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of NIH-3T3 cells using PAK3 Rabbit polyclonal antibody (STJ11101194) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PAK3 antibody (1-100) (STJ11101194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-PAK3 antibody (121-170 aa) (STJ94945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of LNCaP cells, using PAK4 antibody (STJ113249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PAK4 antibody (450-550) (STJ113249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using PAK5 Polyclonal Antibody

Anti-PAK5 antibody (661-710 aa) (STJ94938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using PALD1 Rabbit polyclonal antibody (STJ114759) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PALD1 antibody (1-300) (STJ114759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g PALLD antibody (STJ114240). Western blot was performed from the immunoprecipitate using PALLD antibody (STJ114240) at a dilution of 1:1000.

Anti-PALLD antibody (383-672) (STJ114240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-PALLD antibody (450-530 aa) (STJ94950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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STJ70483 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear, plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-PALS/MPP5 antibody (N-Term) (STJ70483)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC/FC

Reactivity :

Human/Cow/Dog/Pig

£312.50

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Immunohistochemistry analysis of paraffin embedded human esophagus using Pan-CK at dilution of 1:1000

Anti-pan Cytokeratin antibody [SM0456] (STJA0010456)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Immunofluorescence analysis of HeLa TSA and HeLa cells using Pan Acetyl-Lysine Rabbit pAb (STJ111149) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-pan Kac antibody (STJ111149)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Human appendix tissue was stained with Anti-Actin pan (ABT-ACTN) Antibody

Anti-Pan-Actin antibody (300-377 aa) [ABT-ACTN] (STJ196801)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Anti-Pan-Actin antibody (300-377 aa) [PT1737] (STJ196938)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Immunohistochemistry analysis of paraffin embedded human colon using pan actin at dilution of 1:200

Anti-Pan-Actin antibody [SM5131] (STJA0005131)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Anti-Pan-Alpha Tubulin antibody [SM5020] (STJA0005020)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using Cav pan α1Rabbit pAb diluted at 1:200.

Anti-Pan-CACNA1C antibody (1288-1338 aa) (STJ197418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin embedded human pancreas using Pan-cadherin at dilution of 1:200

Anti-Pan-Cadherin antibody [SM4933] (STJA0004933)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Caldesmon (ABT125) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 93kDaObserved band size: 93kDa

Anti-PAN-CALD1 antibody (100-200 aa) [ABT125] (STJ197106)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Immunohistochemistry analysis of paraffin embedded human tonsil using pan Calponin at dilution of 1:200

Anti-Pan-Calponin antibody [SM4934] (STJA0004934)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Anti-Pan-Collagen antibody [SM4985] (STJA0004985)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Pentuple-Fluorescence kit. CK-pan mouse monoclonal antibody (STJ197095) green, Ki-67 rabbit monoclonal antibody (STJA0006295 red), Iba 1 mouse monoclonal antibody (purple), CD8 a mouse monoclonal antibody (cyan), CD20 mouse monoclonal antibody (yellow), The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).

Anti-Pan-Cytokeratin antibody (100-200 aa) [ABT154] (STJ197095)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine Rabbit polyclonal antibody (STJ27698) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Pan-Di-Methyl-Lysine antibody (STJ27698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of Jurkat cells using Keratin-pan Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Pan-KRT2 antibody (151-200 aa) (STJ96694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human lung squamous cell carcinoma (A) , normal hepatocyte (B) , colon adenocacinoma, normal stomach tissue (D) , showing cytoplasmic and membrane localization with DAB staining using Cytokeratin (Pan) Monoc

Anti-Pan-KRT5 antibody [7H8] (STJ97994)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using Cytokeratin (Pan) monoclonal antibody against truncated CK5 recombinant protein.

Anti-Pan-KRT5 antibody [7H8C4] (STJ97993)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of Hela using Pan Methylated Lysine monoclonal antibody.

Anti-Pan-Methyl-Lysine-Mix antibody [Mix] (STJ97446)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Species independent

£48.00 - £252.00

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Western blot analysis of 4T1 cells using MYH-pan Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Pan-MYH-pan antibody (1351-1400 aa) (STJ96792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine pAb (STJ11101040) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Pan-Phospho-Tyrosine antibody (STJ11101040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine Mouse mAb (STJ11101091) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Pan-Phospho-Tyrosine antibody [S1MM] (STJ11101091)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Immunofluorescence analysis of NIH-3T3 cells using pan-PKC Rabbit polyclonal antibody (STJ119908) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Pan-PKC antibody (572-672 aa) (STJ119908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-Pan-PKC antibody [SM5008] (STJA0005008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Anti-Rab8 antibody at 1:500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-Pan-RAB8 antibody (107aa C-Term) (STJ140064)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/ELISA

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Western blot analysis of various cells using Na+ CP-pan Polyclonal Antibody diluted at 1：1000

Anti-Pan-SCN1A/SCN2A/SCN3A/SCN4A/SCN5A/SCN8A/SCN9A/SCN10A/SCN11A antibody (1466-1515) (STJ94335)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ70724 (3µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH6, HRP-staining.

Anti-Pancreatic Polypeptide/PPY antibody (Internal) (STJ70724)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC/FC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Immunofluorescence analysis of MCF7 cells using Pannexin 1 antibody (STJ115548). Blue: DAPI for nuclear staining.

Anti-PANX1 antibody (297-426) (STJ115548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from rat stomach, K562 cells, using PAOX Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PAOX antibody (260-340 aa) (STJ96463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Papillomavirus type 1 E2-protein antibody (182-190 aa) [1E2] (STJA0002810)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF/IP

Reactivity :

PV

£438.50 - £2,502.50

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Anti-Papillomavirus type 1 E2-protein antibody (197-208 aa) [3F12] (STJA0002814)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF

Reactivity :

PV

£438.50 - £2,502.50

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Anti-Papillomavirus type 1 E2-protein antibody (199-208 aa) [5E11] (STJA0002816)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF/IP

Reactivity :

PV

£438.50 - £2,502.50

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Anti-Papillomavirus type 1 E2-protein antibody (208-218 aa) [1H10] (STJA0002812)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF

Reactivity :

PV

£438.50 - £2,502.50

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Anti-Papillomavirus type 1 E2-protein antibody (210-410 aa) [3H5] (STJA0002815)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF

Reactivity :

PV

£438.50 - £2,502.50

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Anti-Papillomavirus type 1 E2-protein antibody (250-280 aa) [1E4] (STJA0002811)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF

Reactivity :

PV

£438.50 - £2,502.50

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