• Immunofluorescence analysis of L929 cells using IDH3B Rabbit polyclonal antibody (STJ115693) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using IDH3B Rabbit polyclonal antibody (STJ115693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from Rat heart, using IDH3B Rabbit polyclonal antibody (STJ115693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IDH3B antibody (35-170) (STJ115693)

SKU:
STJ115693

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IDH3B (35-170) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IDH3B
Gene ID: 3420
Uniprot ID: IDH3B_HUMAN
Immunogen Region: 35-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-170 of human IDH3B (NP_008830.2).
Immunogen Sequence: ASRSQAEDVRVEGSFPVTML PGDGVGPELMHAVKEVFKAA AVPVEFQEHHLSEVQNMASE EKLEQVLSSMKENKVAIIGK IHTPMEYKGELASYDMRLRR KLDLFANVVHVKSLPGYMTR HNNLDLVIIREQTEGE
Function Plays a structural role to facilitate the assembly and ensure the full activity of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
Protein Name Isocitrate Dehydrogenase Nad Subunit Beta - Mitochondrial
Isocitric Dehydrogenase Subunit Beta
Nad(+-Specific Icdh Subunit Beta
Database Links Reactome: R-HSA-71403
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Isocitrate Dehydrogenase Nad Subunit Beta - Mitochondrial antibody
Anti-Isocitric Dehydrogenase Subunit Beta antibody
Anti-Nad(+-Specific Icdh Subunit Beta antibody
Anti-IDH3B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance