• Immunofluorescence analysis of HeLa cells using ID3 antibody (STJ27328).
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using ID3 antibody (STJ27328) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse pancreas using ID3 antibody (STJ27328) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using ID3 antibody (STJ27328) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ID3 antibody (1-119) (STJ27328)

SKU:
STJ27328

£112.50 - £522.50
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Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ID3 (1-119) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ID3
Gene ID: 3399
Uniprot ID: ID3_HUMAN
Immunogen Region: 1-119
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-119 of human ID3 (NP_002158.3).
Immunogen Sequence: MKALSPVRGCYEAVCCLSER SLAIARGRGKGPAAEEPLSL LDDMNHCYSRLRELVPGVPR GTQLSQVEILQRVIDYILDL QVVLAEPAPGPPDGPHLPIQ TAELTPELVISNDKRSFCH
Tissue Specificity Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
Function Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer.
Protein Name Dna-Binding Protein Inhibitor Id-3
Class B Basic Helix-Loop-Helix Protein 25
Bhlhb25
Helix-Loop-Helix Protein Heir-1
Id-Like Protein Inhibitor Hlh 1r21
Inhibitor Of Dna Binding 3
Inhibitor Of Differentiation 3
Database Links Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna-Binding Protein Inhibitor Id-3 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 25 antibody
Anti-Bhlhb25 antibody
Anti-Helix-Loop-Helix Protein Heir-1 antibody
Anti-Id-Like Protein Inhibitor Hlh 1r21 antibody
Anti-Inhibitor Of Dna Binding 3 antibody
Anti-Inhibitor Of Differentiation 3 antibody
Anti-ID3 antibody
Anti-1R21 antibody
Anti-BHLHB25 antibody
Anti-HEIR1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance