• Immunofluorescence analysis of Human-breast tissue. 1, IDE monoclonal antibody (3H4) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of 1) Hela, 2) HepG2, diluted at 1:2000
  • Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, IDE monoclonal antibody (3H4) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IDE antibody [3H4] (STJ96985)

SKU:
STJ96985

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Hamster
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Insulin-degrading enzyme is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 3H4
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:1000
IF 1:200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IDE
Gene ID: 3416
Uniprot ID: IDE_HUMAN
Specificity: The antibody detects endogenous IDE proteins.
Immunogen: Synthetic Peptide of IDE
Post Translational Modifications The N-terminus is blocked.
Function Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin. Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP. Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP. Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients. Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).
Protein Name Insulin-Degrading Enzyme
Abeta-Degrading Protease
Insulin Protease
Insulinase
Insulysin
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-77387
Reactome: R-HSA-9033241
Cellular Localisation Cytoplasm
Cytosol
Cell Membrane
Secreted
Present At The Cell Surface Of Neuron Cells
The Membrane-Associated Isoform Is Approximately 5 Kda Larger Than The Known Cytosolic Isoform
Alternative Antibody Names Anti-Insulin-Degrading Enzyme antibody
Anti-Abeta-Degrading Protease antibody
Anti-Insulin Protease antibody
Anti-Insulinase antibody
Anti-Insulysin antibody
Anti-IDE antibody

Information sourced from Uniprot.org

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