STJ72888 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ICAM1 antibody (Internal) (STJ72888)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ72888-100
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General Information

Short DescriptionGoat polyclonal antibody anti-ICAM1 (Internal) is suitable for use in ELISA and Immunofluorescence research applications.
ApplicationsPep-ELISA/IF
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:4000.
IF: Strong expression of the protein seen in the plasma membrane and cytoplasm of U251 cells. Recommended concentration: 10ยตg/ml.
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolICAM1
Gene ID3383
Uniprot IDICAM1_HUMAN
Accession NumberNP_000192.2
Immunogen RegionInternal
Immunogen SequenceSNCPDGQSTAKT
SpecificityThe immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite.

Additional Info

Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Protein Name Intercellular Adhesion Molecule 1
Icam-1
Major Group Rhinovirus Receptor
Cd Antigen Cd54
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-216083
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-877300
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Intercellular Adhesion Molecule 1 antibody
Anti-Icam-1 antibody
Anti-Major Group Rhinovirus Receptor antibody
Anti-Cd Antigen Cd54 antibody
Anti-ICAM1 antibody

Information sourced from Uniprot.org

Citations

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