| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-IDE (1-250) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:10-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IDE |
| Gene ID: |
3416 |
| Uniprot ID: |
IDE_HUMAN |
| Immunogen Region: |
1-250 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Insulin-degrading enzyme (Insulin-degrading enzyme (IDE) ) (NP_004960.2). |
| Immunogen Sequence: |
MRYRLAWLLHPALPSTFRSV LGARLPPPERLCGFQKKTYS KMNNPAIKRIGNHITKSPED KREYRGLELANGIKVLLISD PTTDKSSAALDVHIGSLSDP PNIAGLSHFCEHMLFLGTKK YPKENEYSQFLSEHAGSSNA FTSGEHTNYYFDVSHEHLEG ALDRFAQFFLCPLFDESCKD REVNAVDSEHEKNVMNDAWR LFQLEKATGNPKHPFSKFGT GNKYTLETRPNQEGIDVRQ |
| Tissue Specificity | Detected in brain and in cerebrospinal fluid (at protein level). |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin. Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP. Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP. Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients. Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV). |
| Protein Name | Insulin-Degrading EnzymeAbeta-Degrading ProteaseInsulin ProteaseInsulinaseInsulysin |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-77387Reactome: R-HSA-9033241 |
| Cellular Localisation | CytoplasmCytosolCell MembraneSecretedPresent At The Cell Surface Of Neuron CellsThe Membrane-Associated Isoform Is Approximately 5 Kda Larger Than The Known Cytosolic Isoform |
| Alternative Antibody Names | Anti-Insulin-Degrading Enzyme antibodyAnti-Abeta-Degrading Protease antibodyAnti-Insulin Protease antibodyAnti-Insulinase antibodyAnti-Insulysin antibodyAnti-IDE antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
