• Immunofluorescence analysis of Jurkat cells using ICOS/CD278 Rabbit polyclonal antibody (STJ24118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HL-60 cells using ICOS/CD278 Rabbit polyclonal antibody (STJ24118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ICOS/CD278 Rabbit polyclonal antibody (STJ24118) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ICOS antibody (21-140) (STJ24118)

SKU:
STJ24118

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ICOS (21-140) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ICOS
Gene ID: 29851
Uniprot ID: ICOS_HUMAN
Immunogen Region: 21-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-140 of human ICOS/CD278 (NP_036224.1).
Immunogen Sequence: EINGSANYEMFIFHNGGVQI LCKYPDIVQQFKMQLLKGGQ ILCDLTKTKGSGNTVSIKSL KFCHSQLSNNSVSFFLYNLD HSHANYYFCNLSIFDPPPFK VTLTGGYLHIYESQLCCQLK
Tissue Specificity Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.
Post Translational Modifications N-glycosylated.
Function Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes.
Protein Name Inducible T-Cell Costimulator
Activation-Inducible Lymphocyte Immunomediatory Molecule
Cd Antigen Cd278
Database Links Reactome: R-HSA-1257604 Q9Y6W8-1
Reactome: R-HSA-2219530 Q9Y6W8-1
Reactome: R-HSA-388841 Q9Y6W8-1
Reactome: R-HSA-6811558 Q9Y6W8-1
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative Antibody Names Anti-Inducible T-Cell Costimulator antibody
Anti-Activation-Inducible Lymphocyte Immunomediatory Molecule antibody
Anti-Cd Antigen Cd278 antibody
Anti-ICOS antibody
Anti-AILIM antibody

Information sourced from Uniprot.org

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