Immunofluorescence Antibodies | St John's Laboratory

STJ70660 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-AKAP9/AKAP450/CG-NAP antibody (C-Term) (STJ70660)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse

£312.50

Choose Options

Western blot analysis of various cells using BCA3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-AKIP1 antibody (111-160 aa) (STJ91827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of HeLa cells using AKR1A1 antibody (STJ115538).

Anti-AKR1A1 antibody (1-325) (STJ115538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Immunofluorescence analysis of PC-12 cells using AKR1B1 Rabbit polyclonal antibody (STJ22567) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-AKR1B1 antibody (1-316) (STJ22567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of various cells using Aldose Reductase Polyclonal Antibody

Anti-AKR1B1 antibody (241-290 aa) (STJ91559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

Choose Options

Anti-AKR1B1 antibody [SRM14490] (STJ11109220)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

Add to Cart The item has been added

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-AKR1C1 antibody (30-110 aa) (STJ92669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of U-2 OS cells using AKR1C2 Polyclonal Antibody (STJ116408) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AKR1C2 antibody (1-323) (STJ116408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of COLO cells using DD2 Polyclonal Antibody

Anti-AKR1C2 antibody (21-70 aa) (STJ92670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of K-562 cells, using 3 Mu g AKR1C3 antibody (STJ22568). Western blot was performed from the immunoprecipitate using AKR1C3 antibody (STJ22568) at a dilution of 1:1000.

Anti-AKR1C3 antibody (1-323) (STJ22568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

Choose Options

Confocal imaging of A549 cells using AKR1C3 Rabbit monoclonal antibody (STJ11101962, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-AKR1C3 antibody (200-300) [S2MR] (STJ11101962)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

Choose Options

Immunofluorescence analysis of A549 cells, using AKR1CL1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AKR1C8 antibody (80-129 aa) (STJ91535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Western blot analysis of various cells using AKR1CL2 Polyclonal Antibody diluted at 1：500

Anti-AKR1E2 antibody (141-190 aa) (STJ91536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of human-stomach tissue. 1, Akt Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Akt antibody (281-330 aa) (STJ91550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£49.00 - £261.00

Choose Options

Western blot analysis of extracts of various cell lines, using AKT1 antibody (STJ27474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1 antibody (100-200) (STJ27474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (STJ112889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-AKT1 antibody (350-480) (STJ112889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Anti-AKT1 antibody (370-477 aa) [10D6] (STJ97724)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/D.melanogaster

£49.00 - £261.00

Choose Options

Confocal imaging of MCF7 cells using [KO Validated] AKT1 Rabbit monoclonal antibody (STJ11104954, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-AKT1 antibody (380-480) [S4954RM] (STJ11104954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

Choose Options

Western blot analysis of lysates from 1) 3T3, 2) 293T, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-AKT1 antibody (400-480 aa) (STJ91540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of human-lung tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-AKT1 antibody (95-144 aa) (STJ91542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

STJ73595 (0. 3µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AKT1 antibody (Internal) (STJ73595)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse

£312.50

Choose Options

Anti-AKT1 nanobody [SAA1231] (STJN000197)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

IF

Reactivity :

Human

£378.50 - £1,632.50

Choose Options

Western blot analysis of 3T3 cells using Akt1/3 Polyclonal Antibody diluted at 1：1000

Anti-Akt1/3 antibody (406-455 aa) (STJ91551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2/3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-AKT1/AKT2/AKT3 antibody (281-480 aa) [R04-8A4] (STJA0013034)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat/Hamster

£264.50

Choose Options

Immunoprecipitation analysis of 25 Mu g extracts of Rat brain cells using 3 Mu g Pan-Akt antibody (STJ11100091). Western blot was performed from the immunoprecipitate using Pan-Akt antibody (STJ11100091) at a dilution of 1:1000.

Anti-AKT1/AKT2/AKT3 antibody (311-480) (STJ11100091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Immunofluorescence analysis of NIH-3T3 cells using AKT1S1/PRAS40 Rabbit polyclonal antibody (STJ27994) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT1S1 antibody (150-256) (STJ27994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of various cells using PRAS40 Polyclonal Antibody

Anti-AKT1S1 antibody (207-256 aa) (STJ95215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of various cells using Akt2 Polyclonal Antibody diluted at 1：500

Anti-AKT2 antibody (432-481 aa) (STJ91552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis using Akt2 monoclonal antibody against A431 (1) , Jurkat (2) , HEK293 (3) , A549 (4) , MCF-7 (5) and PC-12 (6) cell lysate.

Anti-AKT2 antibody [1B6] (STJ97821)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Monkey

£149.00 - £360.00

Choose Options

Anti-AKT2 antibody [SRM14707] (STJ11109437)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

Add to Cart The item has been added

STJ70624 (1µg/ml) staining of HepG2 (A) , Jurkat (B) , Mouse Brain (C) and Rat Brain (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AKT3 antibody (Internal) (STJ70624)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

Choose Options

Anti-AKT3 nanobody [SAA1232] (STJN000317)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

IF

Reactivity :

Human

£378.50 - £1,632.50

Choose Options

Immunofluorescence analysis of HeLa cells using ALAD antibody (STJ114271) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALAD antibody (1-330) (STJ114271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

Choose Options

Immunofluorescence analysis of HeLa cells using ALAS1 antibody (STJ115423). Blue: DAPI for nuclear staining.

Anti-ALAS1 antibody (1-250) (STJ115423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

Choose Options

Western blot analysis of various lysates, using Albumin Rabbit pAb (STJ22574) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ALB antibody (25-609) (STJ22574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Immunofluorescence analysis of HepG2 cells using Albumin Rabbit polyclonal antibody (STJ22575) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALB antibody (25-609) (STJ22575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Anti-Albumin antibody [PT0561R] (STJA0039432)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

Choose Options

Anti-Albumin antibody [SM4927] (STJA0004927)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

Choose Options

Anti-Albumin antibody [SM5071] (STJA0005071)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Rat

£170.50 - £233.50

Choose Options

Anti-Albumin/Alb antibody [R4E22] (STJA0032104)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£367.50

Choose Options

Anti-Albumin/ALB antibody [SRM14727] (STJ11109457)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

Add to Cart The item has been added

Immunohistochemistry analysis of paraffin-embedded human ovary carcinoma (A) , kidney carcinoma (B) , lung carcinoma (C) and breast carcinoma (D) , showing cytoplasmic and membrane localization with DAB staining using CD166 monoclonal antibody.

Anti-ALCAM antibody (405-524 aa) [10F1G12] (STJ97908)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

Choose Options

Western blot analysis of K562 cells using CD166 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ALCAM antibody (481-530 aa) (STJ96761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Aldehyde dehydrogenase 5-A1/SSADH/ALDH5A1 antibody [SRM13425] (STJ11108155)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

Add to Cart The item has been added

Western blot analysis of mouse lung, mouse liver cells using ALDH1A1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ALDH1A1 antibody (21-70 aa) (STJ96522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Anti-ALDH1A1 antibody (250-350 aa) [SRM5086] (STJ11106867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

Choose Options

Western blot analysis of various lysates, using ALDH1A1 rabbit monoclonal antibody (STJ11101231) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALDH1A1 antibody (250-350) [S1MR] (STJ11101231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

Choose Options

Immunofluorescence analysis of HepG2 cells using ALDH1A1 Rabbit polyclonal antibody (STJ22577) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ALDH1A1 antibody (329-345) (STJ22577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options