• Western blot analysis of extracts of various cell lines, using AHCY antibody (STJ27253) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of HepG2 cells using AHCY rabbit polyclonal antibody (STJ27253) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-AHCY antibody (253-432) (STJ27253)

SKU:
STJ27253

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AHCY (253-432) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AHCY
Gene ID: 191
Uniprot ID: SAHH_HUMAN
Immunogen Region: 253-432
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 253-432 of human AHCY (NP_000678.1).
Immunogen Sequence: AMEGYEVTTMDEACQEGNIF VTTTGCIDIILGRHFEQMKD DAIVCNIGHFDVEIDVKWLN ENAVEKVNIKPQVDRYRLKN GRRIILLAEGRLVNLGCAMG HPSFVMSNSFTNQVMAQIEL WTHPDKYPVGVHFLPKKLDE AVAEAHLGKLNVKLTKLTEK QAQYLGMSCDGPFKPDHYRY
Function Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine. Binds copper ions.
Protein Name Adenosylhomocysteinase
Adohcyase
S-Adenosyl-L-Homocysteine Hydrolase
Database Links Reactome: R-HSA-156581
Reactome: R-HSA-1614635
Reactome: R-HSA-2408508
Reactome: R-HSA-5578997
Cellular Localisation Cytoplasm
Melanosome
Nucleus
Endoplasmic Reticulum
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Adenosylhomocysteinase antibody
Anti-Adohcyase antibody
Anti-S-Adenosyl-L-Homocysteine Hydrolase antibody
Anti-AHCY antibody
Anti-SAHH antibody

Information sourced from Uniprot.org

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