• STJ71786 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71786 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71786 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-AGXT/AGT antibody (Internal) (STJ71786)

SKU:
STJ71786-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-AGXT/AGT (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the vesicles of HeLa and HepG2 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:2000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: AGXT
Gene ID: 189
Uniprot ID: AGT1_HUMAN
Immunogen Region: Internal
Accession Number: NP_000021.1
Immunogen Sequence: DKAKKKMYSRK
Function Peroxisomal aminotransferase that catalyzes the transamination of glyoxylate to glycine and contributes to the glyoxylate detoxification. Also catalyzes the transamination between L-serine and pyruvate and contributes to gluconeogenesis from the L-serine metabolism.
Protein Name Alanine--Glyoxylate Aminotransferase
Agt
Serine--Pyruvate Aminotransferase
Spt
Database Links Reactome: R-HSA-389661
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-Alanine--Glyoxylate Aminotransferase antibody
Anti-Agt antibody
Anti-Serine--Pyruvate Aminotransferase antibody
Anti-Spt antibody
Anti-AGXT antibody
Anti-AGT1 antibody
Anti-SPAT antibody

Information sourced from Uniprot.org

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