Anti-AHR antibody (Internal) (STJ71870)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-AHR (Internal) is suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/IHC/IF/FC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:1000.IHC: In paraffin embedded Human Kidney shows both cytoplasm and nuclear staining in DCT. Recommended concentration: 2-4ยตg/ml.IF: Strong expression of the protein seen in the cytoplasm |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | AHR |
| Gene ID | 196 |
| Uniprot ID | AHR_HUMAN |
| Accession Number | NP_001612.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | PENQKHGLNPQSA |
Additional Info
| Post Translational Modifications | Mono-ADP-ribosylated, leading to inhibit transcription activator activity of AHR. |
| Function | Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer. Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation. Xenobiotics can act as ligands: upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists. Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands. Acts as a negative regulator of anti-tumor immunity: indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-BMAL1 heterodimer mediated transcriptional activation of PER1. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription. |
| Protein Name | Aryl Hydrocarbon ReceptorAh ReceptorAhrClass E Basic Helix-Loop-Helix Protein 76Bhlhe76 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-211945Reactome: R-HSA-211976Reactome: R-HSA-211981Reactome: R-HSA-8937144 |
| Cellular Localisation | CytoplasmNucleusInitially CytoplasmicUpon Binding With Ligand And Interaction With A Hsp90It Translocates To The Nucleus |
| Alternative Antibody Names | Anti-Aryl Hydrocarbon Receptor antibodyAnti-Ah Receptor antibodyAnti-Ahr antibodyAnti-Class E Basic Helix-Loop-Helix Protein 76 antibodyAnti-Bhlhe76 antibodyAnti-AHR antibodyAnti-BHLHE76 antibody |
Information sourced from Uniprot.org