• STJ73111 (0. 1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73111 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ73111 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73111 (5µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
  • STJ73111 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Anti-AIFM1 antibody (183-195) (STJ73111)

SKU:
STJ73111-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC
Reactivity: Human/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-AIFM1 (183-195) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
IHC-5-7µg/ml
IF-Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and NIH3T3 cells. 5-10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: AIFM1
Gene ID: 9131
Uniprot ID: AIFM1_HUMAN
Immunogen Region: 183-195
Accession Number: NP_004199.1; NP_665811.1; NP_001124319.1
Specificity: This antibody is expected to recognize isoform 1 (NP_004199.1) , isoform 2 (NP_665811.1) and isoform 5 (NP_001124319).
Immunogen Sequence: DDPNVTKTLRFKQ
Post Translational Modifications Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner. Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation. Ubiquitination at Lys-255 by XIAP/BIRC4 blocks its ability to bind DNA and induce chromatin degradation, thereby inhibiting its ability to induce cell death.
Function Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Binds to DNA in a sequence-independent manner. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import. Isoform 4: Has NADH oxidoreductase activity. Does not induce nuclear apoptosis. Isoform 5: Pro-apoptotic isoform.
Protein Name Apoptosis-Inducing Factor 1 - Mitochondrial
Programmed Cell Death Protein 8
Cellular Localisation Mitochondrion Intermembrane Space
Mitochondrion Inner Membrane
Cytoplasm
Nucleus
Perinuclear Region
Proteolytic Cleavage During Or Just After Translocation Into The Mitochondrial Intermembrane Space (Ims) Results In The Formation Of An Inner-Membrane-Anchored Mature Form (Aifmit)
During Apoptosis
Further Proteolytic Processing Leads To A Mature Form
Which Is Confined To The Mitochondrial Ims In A Soluble Form (Aifsol)
Aifsol Is Released To The Cytoplasm In Response To Specific Death Signals
And Translocated To The Nucleus
Where It Induces Nuclear Apoptosis
Release Into The Cytoplasm Is Mediated Upon Binding To Poly-Adp-Ribose Chains
Translocation Into The Nucleus Is Promoted By Interaction With (Auto-Poly-Adp-Ribosylated) Processed Form Of Parp1
Colocalizes With Eif3g In The Nucleus And Perinuclear Region
Isoform 3: Mitochondrion Intermembrane Space
Has A Stronger Membrane Anchorage Than Isoform 1
Isoform 4: Mitochondrion
Cytosol
In Pro-Apoptotic Conditions
Is Released From Mitochondria To Cytosol In A Calpain/Cathepsin-Dependent Manner
Isoform 5: Cytoplasm
Alternative Antibody Names Anti-Apoptosis-Inducing Factor 1 - Mitochondrial antibody
Anti-Programmed Cell Death Protein 8 antibody
Anti-AIFM1 antibody
Anti-AIF antibody
Anti-PDCD8 antibody

Information sourced from Uniprot.org

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