Immunocytochemistry Antibodies | St John's Laboratory

Western blot analysis of 293F, using S100A12 antibody (STJ27281) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-S100A12 antibody (1-92) (STJ27281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using FSP1/S100A4 Rabbit polyclonal antibody (STJ11103860) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-S100A4 antibody (1-101) (STJ11103860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of FSP1/S100A4 in paraffin-embedded human liver using FSP1/S100A4 Rabbit polyclonal antibody (STJ11104557) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-S100A4 antibody (1-101) (STJ11104557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of paraffin-embedded Human colon using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-S100A4 antibody (2-101) [S6MR] (STJ11101646)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from U-251 MG cells using S100A6 Rabbit pAb (STJ11106830) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-S100A6 antibody (1-90 aa) (STJ11106830)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using [KO Validated] S100A6 Rabbit polyclonal antibody (STJ25432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-S100A6 antibody (1-90) (STJ25432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Confocal imaging of C2C12 cells using S100A6 Rabbit monoclonal antibody (STJ11101874, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-S100A6 antibody (1-90) [S4MR] (STJ11101874)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Confocal immunofluorescence analysis of U-2 OS cells using S100A8 Polyclonal Antibody (STJ117510) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-S100A8 antibody (1-93) (STJ117510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded human skin cancer using S100A8 Rabbit polyclonal antibody (STJ25434) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-S100A8 antibody (1-93) (STJ25434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-S100A9 antibody (1-113 aa) [SRM] (STJ11107246)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from Mouse spleen using S100A9 Rabbit pAb (STJ111884) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-S100A9 antibody (20-114) (STJ111884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g S100B antibody (STJ29811). Western blot was performed from the immunoprecipitate using S100B antibody (STJ29811) at a dilution of 1:500.

Anti-S100B antibody (1-92) (STJ29811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of Rat brain, using S100B rabbit monoclonal antibody (STJ11101645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-S100B antibody (1-92) [S5MR] (STJ11101645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates using S100P Rabbit pAb (STJ116980) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-S100P antibody (1-95) (STJ116980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-S100P antibody (C-Term) (STJ502848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£466.50

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Anti-S100P antibody (C-Term) {Biotin} (STJ502851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Anti-S100P antibody (C-Term) {FITC} (STJ502852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Western blot with Anti-S100P Antibody (STJ502846) with S100B peptide. Antibody at 1:500 dilution in buffer. 15% discontinuous gel.

Anti-S100P antibody (Full Length) (STJ502846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£466.50

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Anti-S100P antibody (Full Length) {Biotin} (STJ502849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-S100P antibody (Full Length) {FITC} (STJ502850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-S100P antibody (N-Term) (STJ502847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£466.50

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Anti-S100P antibody (N-Term) {Biotin} (STJ502853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Anti-S100P antibody (N-Term) {FITC} (STJ502854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Western blot analysis of various lysates using SAFB Rabbit pAb (STJ110236) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-SAFB antibody (1-200) (STJ110236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot detection of SAFB1 in HelaNE, A431, MDA-MB-468, Breast cancer, ZR75-1 and MCF7 cell lysates using SAFB1 mouse mAb (1:4000 diluted).Predicted band size: 130kDa.Observed band size: 130kDa.

Anti-SAFB antibody [2E8-E2-G6] (STJ99122)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various lysates using Sall4 Rabbit pAb (STJ29204) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 90s.

Anti-SALL4 antibody (1-220) (STJ29204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded rat testis using Sall4 Rabbit polyclonal antibody (STJ118633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SALL4 antibody (853-1053) (STJ118633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of SAMD9 in A549 lysates using SAMD9 antibody.

Anti-SAMD9 antibody (84-189 aa) [R09-5B4] (STJA0011932)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£264.50

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Western blot analysis of various lysates, using SARDH Rabbit pAb (STJ113655) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 40s.

Anti-SARDH antibody (300-560) (STJ113655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-SARM1 antibody (Full Length) (STJA0004904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£454.50

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Immunofluorescence analysis of U-2 OS cells using SARNP Rabbit polyclonal antibody (STJ115656) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SARNP antibody (1-210) (STJ115656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of MCF7 cells using SARS antibody (STJ28816). Blue: DAPI for nuclear staining.

Anti-SARS antibody (1-300) (STJ28816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein, using SARS-CoV-2 Nucleoprotein antibody (STJ11101492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SARS-CoV-2 Nucleoprotein antibody (1-420) (STJ11101492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IF/ICC/IP/ELISA

Reactivity :

SARS-CoV-2

£146.50 - £642.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike antibody (STJ11101794). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike antibody (STJ11101794) at a dilution of 1:1000.

Anti-SARS-CoV-2 Spike antibody (600-700) (STJ11101794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IF/ICC/IP/ELISA

Reactivity :

SARS-CoV-2

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike RBD antibody (STJ11101792). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike RBD antibody (STJ11101792) at a dilution of 1:3000.

Anti-SARS-CoV-2 Spike RBD antibody (332-550) (STJ11101792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

SARS-CoV-2

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike S1 antibody (STJ11101793). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S1 antibody (STJ11101793) at a dilution of 1:10000.

Anti-SARS-CoV-2 Spike S1 antibody (11-682) (STJ11101793)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/SARS-CoV-2

£112.50 - £524.50

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Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 3CLpro fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 3CLpro Rabbit polyclonal antibody (STJ11103221) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SARS-CoV-2-3CLpro antibody (1-306) (STJ11103221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

SARS-CoV-2

£112.50 - £524.50

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Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 N Protein Mouse monoclonal antibody (STJ11103176) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SARS-CoV-2-N Protein antibody (1-420 aa) [S6MM] (STJ11103176)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

SARS-CoV-2

£129.50 - £574.50

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Immunofluorescence analysis of 293T-SARS-CoV-2 NSP13 (His-tag) and 293T cells using SARS-CoV-2 NSP13 Rabbit polyclonal antibody (STJ11103267) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SARS-CoV-2-NSP13 antibody (1-601) (STJ11103267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

SARS-CoV-2

£112.50 - £524.50

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Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with B-tubulin as a loading control.

Anti-SARS-CoV-2-S-Protein ACE 2 Binding Domain antibody (STJA0003863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

£454.50

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Anti-SARS-CoV-2-S-Protein ACE 2 Binding Domain antibody [2G1] (STJA0003861)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

£454.50

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Anti-SARS-CoV-2-S-Protein ACE 2 Binding Domain antibody [5G8] (STJA0003862)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

£454.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SARS-CoV-2 Spike RBD using SARS-CoV-2 Spike RBD Mouse mAb (STJ11106956) at 1:500 dilution. <br/>Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-SARS-CoV-2-Spike RBD antibody [SMM] (STJ11106956)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/DB/IF/ICC/ELISA

Reactivity :

SARS-CoV-2

£129.50 - £574.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike S2 ECD antibody (STJ11103173). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 ECD antibody (STJ11103173) at a dilution of 1:5000.

Anti-SARS-CoV-2-Spike S2 ECD antibody (686-1214) (STJ11103173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IF/ICC/IP/ELISA

Reactivity :

Human/SARS-CoV-2

£146.50 - £642.50

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Western blot analysis of extracts of Jurkat cells, using SATB1 antibody (STJ28364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SATB1 antibody (572-763) (STJ28364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of SBDS (green) in Hela using SBDS antibody, and DAPI (blue)

Anti-SBDS antibody (237-250 aa) [R02-2G2] (STJA0012583)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£264.50

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STJ71003 staining of HFF cell lysate with B) 100nM siRNA or A) control siRNA. Detected by chemiluminescence. Data kindly provided by Laura Papp and Kum Kum Khanna, Brisbane, Australia.

Anti-SBP2 antibody (Internal) (STJ71003)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IP/IHC/ICC/IF

Reactivity :

Human/Dog/Pig/Cow

£312.50

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Immunofluorescence analysis of U-2 OS cells using SCAMP2 Rabbit polyclonal antibody (STJ119669) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SCAMP2 antibody (1-152) (STJ119669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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