• Western blot analysis of extracts of various cell lines, using LTA4H rabbit monoclonal antibody (STJ11102395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Confocal imaging of C6 cells using LTA4H Rabbit monoclonal antibody (STJ11102395, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of C6 cells using LTA4H rabbit monoclonal antibody (STJ11102395) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal imaging of NIH/3T3 cells using LTA4H Rabbit monoclonal antibody (STJ11102395, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo®488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of NIH-3T3 cells using LTA4H rabbit monoclonal antibody (STJ11102395) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using LTA4H Rabbit monoclonal antibody (STJ11102395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-LTA4H antibody (501-600) [S5MR] (STJ11102395)

SKU:
STJ11102395

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-LTA4H (501-600) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LTA4H
Gene ID: 4048
Uniprot ID: LKHA4_HUMAN
Immunogen Region: 501-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 501-600 of human LTA4H (P09960).
Immunogen Sequence: NEFLAQTLQRAPLPLGHIKR MQEVYNFNAINNSEIRFRWL RLCIQSKWEDAIPLALKMAT EQGRMKFTRPLFKDLAAFDK SHDQAVRTYQEHKASMHPVT
Tissue Specificity Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts.
Post Translational Modifications Phosphorylation at Ser-416 inhibits leukotriene-A4 hydrolase activity.
Function Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides. In addition to its pro-inflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions.
Protein Name Leukotriene A-4 Hydrolase
Lta-4 Hydrolase
Leukotriene A(4 Hydrolase
Tripeptide Aminopeptidase Lta4h
Database Links Reactome: R-HSA-2142691
Reactome: R-HSA-6798695
Reactome: R-HSA-9018676
Reactome: R-HSA-9018681
Reactome: R-HSA-9018896
Reactome: R-HSA-9020265
Reactome: R-HSA-9023661
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Leukotriene A-4 Hydrolase antibody
Anti-Lta-4 Hydrolase antibody
Anti-Leukotriene A(4 Hydrolase antibody
Anti-Tripeptide Aminopeptidase Lta4h antibody
Anti-LTA4H antibody
Anti-LTA4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance