Anti-Lysosomal associated membrane protein 1 antibody [LAMP1] (STJA0040770)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenRecombinant LAMP1 expressed and purified from E. coli.
General Information
| Short Description | Mouse monoclonal anti-Lysosomal associated membrane protein 1 for use in ICC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | LAMP1 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | IgG |
| Dilution Range | WB 1:5000-1:10000 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at-20°C for |
Target Information
| Gene Symbol | LAMP1 |
| Gene ID | 3916 |
| Uniprot ID | LAMP1_HUMAN |
| Immunogen | Recombinant LAMP1 expressed and purified from E. coli. |
| Immunogen Sequence | Human |
| Specificity | The antibody reacts with a diffuse band at ~90 kDa to 120 kDa by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry. |
Additional Info
| Post Translational Modifications | O- and N-glycosylated.some of the 18 N-linked glycans are polylactosaminoglycans. (Microbial infection) The glycosylation of Asn-76 is essential for Lassa virus entry into cells. |
| Function | Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Also plays an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins. (Microbial infection) Acts as a receptor for Lassa virus glycoprotein. Also promotes fusion of the virus with host membrane in less acidic endosomes. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F. |
| Protein Name | Lysosome-Associated Membrane Glycoprotein 1Lamp-1Lysosome-Associated Membrane Protein 1Cd107 Antigen-Like Family Member ACd Antigen Cd107a |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Lysosome MembraneSingle-Pass Type I Membrane ProteinEndosome MembraneLate Endosome MembraneCell MembraneCytolytic Granule MembraneThis Protein Shuttles Between LysosomesEndosomesAnd The Plasma MembraneColocalizes With Osbpl1a At The Late Endosome |
| Alternative Antibody Names | Anti-Lysosome-Associated Membrane Glycoprotein 1 antibodyAnti-Lamp-1 antibodyAnti-Lysosome-Associated Membrane Protein 1 antibodyAnti-Cd107 Antigen-Like Family Member A antibodyAnti-Cd Antigen Cd107a antibodyAnti-LAMP1 antibody |
Information sourced from Uniprot.org