Anti-Lysophospholipase 1 antibody [R03-3R1] (STJA0037056)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Lysophospholipase 1
STJA0037056
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General Information

Short DescriptionRabbit monoclonal anti-Lysophospholipase 1 for use in WB, ICC, IF and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ICC/IF/FC
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR03-3R1
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Chromatography
Dilution RangeWB 1:500-1:1000
IF 1:50-1:200
FC 1:50-1:100
FormulationRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolLYPLA1
Gene ID10434
Uniprot IDLYPA1_HUMAN
ImmunogenA synthesized peptide derived from human Lysophospholipase 1

Additional Info

Tissue Specificity Platelets.
Function Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA.
Protein Name Acyl-Protein Thioesterase 1
Apt-1
Hapt1
Lysophospholipase 1
Lysophospholipase I
Lpl-I
Lysopla I
Palmitoyl-Protein Hydrolase
Database Links Reactome: R-HSA-203615
Reactome: R-HSA-9648002
Cellular Localisation Cytoplasm
Cell Membrane
Nucleus Membrane
Endoplasmic Reticulum
Shows Predominantly A Cytoplasmic Localization With A Weak Expression In The Cell Membrane
Nuclear Membrane And Endoplasmic Reticulum
Alternative Antibody Names Anti-Acyl-Protein Thioesterase 1 antibody
Anti-Apt-1 antibody
Anti-Hapt1 antibody
Anti-Lysophospholipase 1 antibody
Anti-Lysophospholipase I antibody
Anti-Lpl-I antibody
Anti-Lysopla I antibody
Anti-Palmitoyl-Protein Hydrolase antibody
Anti-LYPLA1 antibody
Anti-APT1 antibody
Anti-LPL1 antibody

Information sourced from Uniprot.org

Citations

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