ELISA Antibodies | St John's Laboratory

Western blot analysis of lysates from HeLa cells, using Beta-Actin Mouse mAb (STJ11103683) at 1:2000-1:640000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Beta-Actin antibody [S3MR] (STJ11103683)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£135.50 - £249.50

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Immunohistochemistry analysis of Beta-Catenin in paraffin-embedded human skin using Beta-Catenin Mouse monoclonal antibody (STJ11103229) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Beta-Catenin antibody (300-400 aa) [S9MM] (STJ11103229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates using Beta-Tubulin Mouse mAb (STJ11107557) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Beta-Tubulin antibody [SMM] (STJ11107557)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Hamster/Monkey/Goat/C.reinhardtii

£160.50 - £300.50

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Western blot analysis of various lysates using HRP-conjugated Beta-Tubulin Mouse mAb (STJ11107559) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Beta-Tubulin antibody [SMM] {HRP} (STJ11107559)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£173.50 - £325.50

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Anti-Beta1-Integrin antibody [DF5] (STJ16100803)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IHC-F/WB/ELISA

Reactivity :

Human

£685.50

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Anti-Beta1-Integrin antibody [DF7] (STJ16100804)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IHC-F/WB/ELISA

Reactivity :

Human

£685.50

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Anti-Beta3-Integrin antibody [BB10] (STJ16100812)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB/ELISA

Reactivity :

Human

£606.50

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HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse ßCstf-64 (DYKDDDDK tagged) immunoprecipitated using STJ72911 (2ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDDDDK (1/1000). Mock-transfected HeLa in Lane 1. A 1/40th part of the lysate was loaded in lanes 2 and 3 before IP and in lanes 4 an 5 after IP. Primary incubations were for 1 hour. Detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.

Anti-betaCstF-64 variant 1/betaCstF-64 variant 3-Mouse antibody (Internal) (STJ72911)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IP

Reactivity :

Mouse

£312.50

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Anti-BETVII/Allergen Bet v II antibody [1A448] (STJA0022362)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

B.pendula

£229.50 - £328.50

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Anti-BETVII/Allergen Bet v II antibody [SAA0670] (STJA0019489)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

B.pendula

£378.50 - £1,632.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BEX3 antibody (1-80 aa) (STJ192330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using BFAR Rabbit pAb (STJ110606) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-BFAR antibody (1-140) (STJ110606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using BG1 Rabbit polyclonal antibody (STJ11104065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-BG1 antibody (STJ11104065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

O.sativa

£146.50 - £642.50

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Western blot analysis of Rat lung, using Osteocalcin (BGLAP) rabbit polyclonal antibody (STJ116843) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BGLAP antibody (1-100) (STJ116843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of U-87MG cells, using Osteocalcin (BGLAP) antibody (STJ27961) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BGLAP antibody (1-100) (STJ27961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Osteocalcin (BGLAP) antibody (STJ11100198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BGLAP antibody (1-95) (STJ11100198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BGLAP antibody (31-80 aa) (STJ192217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry of rat tissue with Anti-BGLAP primary antibody (STJ502017) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-BGLAP antibody (50-100) (STJ502017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Pig/Rat

£427.50

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Anti-BGLAP antibody (50-100) {Biotin} (STJ502018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Pig/Rat

£528.50

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Anti-BGLAP antibody (50-100) {FITC} (STJ502019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Pig/Rat

£528.50

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Western blot analysis of various lysates using BGN Rabbit polyclonal antibody (STJ116192) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-BGN antibody (20-230) (STJ116192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of HCT 116 cells using BGN Rabbit polyclonal antibody (STJ28337) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BGN antibody (38-368) (STJ28337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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STJ73539 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-BHLHE22 antibody (128-141) (STJ73539)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Pig

£312.50

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STJ73538 (0. 1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-BHLHE22 antibody (Internal) (STJ73538)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of extracts of various cell lines, using BHLHE40 antibody (STJ28617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BHLHE40 antibody (121-412) (STJ28617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from RAW264.7 cells, using BHLHB3 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-BHLHE41 antibody (1-50 aa) (STJ92684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates, using BHLHE41 Rabbit polyclonal antibody (STJ112573) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BHLHE41 antibody (120-280) (STJ112573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Rat liver, using BHMT rabbit polyclonal antibody antibody (STJ22796) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BHMT antibody (177-406) (STJ22796)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using BHMT Rabbit monoclonal antibody (STJ11102269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180S.

Anti-BHMT antibody (300-406) [S9MR] (STJ11102269)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ71251 (0. 03µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-BHMT antibody (C-Term) (STJ71251)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Western blot analysis using BHMT monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY BHMT cDNA (2).

Anti-BHMT antibody [8C11H5] (STJ97863)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-BHMT2 antibody (STJA0004764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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Anti-BHMT2 antibody (STJA0004765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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HEK293 overexpressing Human BICC1 with N-terminal FLAG, probed with rabbit anti-BCC1 in Western Blot after IP using either STJ72131 or anti-FLAG antibody in the presence or absence of SDS.

Anti-BICC1 antibody (223-234) (STJ72131)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IP

Reactivity :

Human/Rat/Dog/Cow

£312.50

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Western blot analysis of extracts of various cell lines, using BICD2 antibody (STJ112330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BICD2 antibody (440-620) (STJ112330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BICRA antibody (STJA0004838)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

£140.50 - £415.50

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Anti-BICRA antibody (STJA0004839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

£140.50 - £415.50

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Immunofluorescence analysis of NIH-3T3 cells using Bid Rabbit polyclonal antibody (STJ22797) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Bid antibody (1-195) (STJ22797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and Bid Rabbit monoclonal antibody knockdown (KD) 293T (KD) cells, using Bid Rabbit monoclonal antibody (STJ11105057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BID antibody (1-195) [S5057RM] (STJ11105057)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of various cells using BID Polyclonal Antibody diluted at 1：1000

Anti-BID antibody (44-93 aa) (STJ91853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ70684 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-BID antibody (C-Term) (STJ70684)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human

£312.50

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Western blot analysis using BID monoclonal antibody against HeLa (1) , A431 (2) , Jurkat (3) , A549 (4) , HepG2 (5) , and HEK293 (6) cell lysate.

Anti-BID antibody [3C5] (STJ97864)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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STJ70058 staining (0. 1µg/ml) of HEK293 (A) and A431 (B) cell lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.

Anti-BIF-1/SH3GLB1 antibody (C-Term) (STJ70058)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-Big Endothelin1/Big-ET-1 antibody [SAA0803] (STJA0019619)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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STJ71039 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Biglycan Preproprotein antibody (C-Term) (STJ71039)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Western blot analysis of lysates from Raji cells, using BIK Rabbit pAb (STJ11107023). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-BIK antibody (1-130 aa) (STJ11107023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using NBK Polyclonal Antibody diluted at 1：500

Anti-BIK antibody (18-67) (STJ94348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ70563 staining (0. 5µg/ml) of K562 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-BIM antibody (C-Term) (STJ70563)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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