Anti-BCL10 antibody (1-233) [S3MR] (STJ11102093)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Bcl10 (1-233) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S3MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCL10 |
| Gene ID | 8915 |
| Uniprot ID | BCL10_HUMAN |
| Immunogen Region | 1-233 |
| Immunogen Sequence | MEPTAPSLTEEDLTEVKKDA LENLRVYLCEKIIAERHFDH LRAKKILSREDTEEISCRTS SRKRAGKLLDYLQENPKGLD TLVESIRREKTQNFLIQKIT DEVLKLRNIKLEHLKGLKCS SCEPFPDGATNNLSRSNSDE SNFSEKLRASTVMYHPEGES STTPFFSTNSSLNLPVLEVG RTENTIFSSTTLPRPGDPGA PPLPPDLQLEEEGTCANSSE MFLPLRSRTVSRQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human Bcl10 (O95999). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. Phosphorylated by IKBKB/IKKB. Ubiquitinated via both 'Lys-63'-linked and linear ('Met-1'-linked) polyubiquitin chains in response to T-cell receptor (TCR) activation. Ubiquitination is recognized by IKBKG/NEMO, the regulatory subunit of I-kappa-B kinase (IKK), and is required for TCR-induced NF-kappa-B activation. Linear ubiquitination at Lys-17, Lys-31 and Lys-63 is mediated by RNF31/HOIP.linear ubiquitination is recognized with much higher affinity than 'Lys-63'-linked ubiquitin by IKBKG/NEMO. CARD11 is required for linear ubiquitination by HOIP by promoting the targeting of BCL10 to RNF31/HOIP. Proteolytically cleaved by MALT1.required for T-cell activation. |
| Function | Plays a key role in both adaptive and innate immune signaling by bridging CARD domain-containing proteins to immune activation. Acts by channeling adaptive and innate immune signaling downstream of CARD domain-containing proteins CARD9, CARD11 and CARD14 to activate NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Recruited by activated CARD domain-containing proteins: homooligomerized CARD domain-containing proteins form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10, subsequent recruitment of MALT1 and formation of a CBM complex. This leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Activated by CARD9 downstream of C-type lectin receptors.CARD9-mediated signals are essential for antifungal immunity. Activated by CARD11 downstream of T-cell receptor (TCR) and B-cell receptor (BCR). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. |
| Protein Name | B-Cell Lymphoma/Leukemia 10B-Cell Cll/Lymphoma 10Bcl-10Card-Containing Molecule Enhancing Nf-Kappa-BCard-Like Apoptotic ProteinHclapCed-3/Ich-1 Prodomain Homologous E10-Like RegulatorCiperCellular Homolog Of VcarmenCcarmenCellular-E10C-E10Mammalian Card-Containing Adapter Molecule E10Me10 |
| Database Links | Reactome: R-HSA-1169091Reactome: R-HSA-202424Reactome: R-HSA-2871837Reactome: R-HSA-5607764Reactome: R-HSA-8866654 |
| Cellular Localisation | CytoplasmPerinuclear RegionMembrane RaftAppears To Have A PerinuclearCompact And Filamentous Pattern Of ExpressionAlso Found In The Nucleus Of Several Types Of Tumor CellsColocalized With Dpp4 In Membrane Rafts |
| Alternative Antibody Names | Anti-B-Cell Lymphoma/Leukemia 10 antibodyAnti-B-Cell Cll/Lymphoma 10 antibodyAnti-Bcl-10 antibodyAnti-Card-Containing Molecule Enhancing Nf-Kappa-B antibodyAnti-Card-Like Apoptotic Protein antibodyAnti-Hclap antibodyAnti-Ced-3/Ich-1 Prodomain Homologous E10-Like Regulator antibodyAnti-Ciper antibodyAnti-Cellular Homolog Of Vcarmen antibodyAnti-Ccarmen antibodyAnti-Cellular-E10 antibodyAnti-C-E10 antibodyAnti-Mammalian Card-Containing Adapter Molecule E10 antibodyAnti-Me10 antibodyAnti-BCL10 antibodyAnti-CIPER antibodyAnti-CLAP antibody |
Information sourced from Uniprot.org