Anti-BCL2L11 antibody (30-130) (STJ114428)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Bim (30-130) antibody for use in IHC-P and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCL2L11 |
| Gene ID | 10018 |
| Uniprot ID | B2L11_HUMAN |
| Immunogen Region | 30-130 |
| Immunogen Sequence | PGAPTSLQTEPQGNPEGNHG GEGDSCPHGSPQGPLAPPAS PGPFATRSPLFIFMRRSSLL SRSSSGYFSFDTDRSPAPMS CDKSTQTPSPPCQAFNHYLS A |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 30-130 of human Bim (NP_619527.1). |
Additional Info
| Tissue Specificity | Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. |
| Post Translational Modifications | Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation. Deubiquitination catalyzed by USP27X stabilizes the protein. Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein. |
| Function | Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis. |
| Protein Name | Bcl-2-Like Protein 11Bcl2-L-11Bcl2-Interacting Mediator Of Cell Death |
| Database Links | Reactome: R-HSA-111446Reactome: R-HSA-111453Reactome: R-HSA-193648Reactome: R-HSA-6802952Reactome: R-HSA-8862803Reactome: R-HSA-8952158Reactome: R-HSA-9607240Reactome: R-HSA-9614657 |
| Cellular Localisation | Endomembrane SystemPeripheral Membrane ProteinAssociated With Intracytoplasmic MembranesIsoform Bimel: MitochondrionTranslocates From Microtubules To Mitochondria On Loss Of Cell AdherenceIsoform Biml: MitochondrionIsoform Bims: MitochondrionIsoform Bim-Alpha1: Mitochondrion |
| Alternative Antibody Names | Anti-Bcl-2-Like Protein 11 antibodyAnti-Bcl2-L-11 antibodyAnti-Bcl2-Interacting Mediator Of Cell Death antibodyAnti-BCL2L11 antibodyAnti-BIM antibody |
Information sourced from Uniprot.org