ELISA Antibodies | St John's Laboratory

STJ71724 (1µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOL5 antibody (C-Term) (STJ71724)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of extracts from K562 cells, using ApoL6 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-APOL6 antibody (240-320 aa) (STJ96424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ71653 (1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOL6 antibody (Internal) (STJ71653)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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STJ71725 (2µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOL6 antibody (Internal) (STJ71725)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Anti-APOLD1/VERGE antibody (C-Term) (STJ71655)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/FC

Reactivity :

Human

£312.50

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STJ71719 (2µg/ml) staining of paraffin embedded Mouse Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-apolipoprotein A-IV-Mouse antibody (Internal) (STJ71719)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Mouse/Rat

£312.50

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Anti-Apolipoprotein C-III antibody (Internal) (STJ73798)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

£312.50

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Immunofluorescence analysis of paraffin-embedded mouse liver using Apolipoprotein C3 Rabbit monoclonal antibody (STJ11103477) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Apolipoprotein C3 antibody (1-99) [S7MR] (STJ11103477)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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STJ71651 (0. 5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Apolipoprotein F antibody (C-Term) (STJ71651)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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Anti-apolipoprotein L6-Mouse antibody (Internal) (STJ71654)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse

£312.50

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STJ71656 (0. 5µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Apolipoprotein M-Mouse antibody (Internal) (STJ71656)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Mouse/Rat

£312.50

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Confocal imaging of paraffin-embedded Human liver cancer tissue using ApoM Rabbit monoclonal antibody (STJ11102273, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-APOM antibody (1-100) [S3MR] (STJ11102273)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunofluorescence analysis of L929 cells using ApoM Rabbit polyclonal antibody (STJ27289) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-APOM antibody (22-188) (STJ27289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ71072 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-APOM antibody (C-Term) (STJ71072)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Dog

£312.50

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Western blot analysis using ApoM monoclonal antibody against GST-ApoM recombinant protein (1) and human serum (2).

Anti-APOM antibody [10C3G5] (STJ97842)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using ApoM monoclonal antibody against human serum (1, 2).

Anti-APOM antibody [8F12C6B8] (STJ97841)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using ApoO monoclonal antibody against HepG2 (1) and 3T3L1 (2) cell lysate

Anti-APOO antibody [2F1] (STJ97843)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis of SH-SY5Y 293T 3T3 lysis using ApoOL antibody. Antibody was diluted at 1:1000

Anti-APOOL antibody (219-268 aa) (STJ91647)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using APP antibody (STJ112892) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-APP antibody (1-100) (STJ112892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of U-87MG cells, using APP antibody (STJ118700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-APP antibody (1-100) (STJ118700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from SKOV3 cells, using APP Rabbit polyclonal antibody (STJ22657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APP antibody (1-100) (STJ22657)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human esophagus using APP antibody (STJ22656) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-APP antibody (200-300) (STJ22656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-amyloid-Alpha-beta (PTR2007) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: SH-SY5YPredicted band size: 86kDaObserved band size: 95kDa

Anti-APP antibody (200-300) [PTR2007] (STJA0006225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Amyloid-Beta Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-APP antibody (221-270 aa) (STJ98585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 3T3 Hela cells using Amyloid-Beta Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-APP antibody (631-680 aa) (STJ98604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of SH-SY5Y cells using APP Rabbit polyclonal antibody (STJ11103984) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-APP antibody (671-770) (STJ11103984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g [KO Validated] APP Rabbit monoclonal antibody (STJ11101455). Western blot was performed from the immunoprecipitate using [KO Validated] APP Rabbit monoclonal antibody (STJ11101455) at a dilution of 1:1000.

Anti-APP antibody (671-770) [S5MR] (STJ11101455)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-APP antibody (711-760 aa) (STJ91591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using APP monoclonal antibody against truncated APP recombinant protein.

Anti-APP antibody [1B11F3] (STJ97844)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various lysates using APPBP2 Rabbit polyclonal antibody (STJ118226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 35s.

Anti-APPBP2 antibody (386-585) (STJ118226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293 using APP-BP2 antibody.. Secondary antibody was diluted at 1:20000

Anti-APPBP2 antibody (390-470 aa) (STJ97627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ70418 (0. 3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APPL/DIP13alpha antibody (C-Term) (STJ70418)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of extracts of various cell lines, using APPL1 antibody (STJ22658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APPL1 antibody (1-210) (STJ22658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using APPL1 Polyclonal Antibody

Anti-APPL1 antibody (121-170 aa) (STJ91648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using APPL11 Rabbit mAb (STJ11102397) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-APPL1 antibody (350-450) [S7MR] (STJ11102397)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ71081 (0. 5µg/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after infection with Human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.

Anti-APPL1 antibody (Internal) (STJ71081)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis of various lysates using APPL2 Rabbit polyclonal antibody (STJ116800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APPL2 antibody (1-300) (STJ116800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using APRR3 Rabbit polyclonal antibody (STJ11104778) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-APRR3 antibody (245-371 aa) (STJ11104778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana

£146.50 - £642.50

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Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using APRR7 Rabbit polyclonal antibody (STJ11104779) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-APRR7 antibody (236-339 aa) (STJ11104779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana

£146.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using APRT Rabbit polyclonal antibody (STJ115881) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-APRT antibody (1-180) (STJ115881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293T, using CALCA Rabbit polyclonal antibody (STJ27409) at 1:2500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-APRT antibody (1-180) (STJ27409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-APTX antibody (11-60 aa) (STJ192599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 150 Mu g extracts of A549 cells using 3 Mu g APTX antibody (STJ27317). Western blot was performed from the immunoprecipitate using APTX antibody (STJ27317) at a dilution of 1:500.

Anti-APTX antibody (93-342) (STJ27317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human

£112.50 - £524.50

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STJ73285 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Anti-APXL antibody (C-Term) (STJ73285)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Cow

£312.50

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Western blot analysis of lysates from rat brain cells, using AQP1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-AQP1 antibody (101-150 aa) (STJ91652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Confocal imaging of paraffin-embedded Mouse kidney tissue using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-AQP1 antibody (170-269) [S5MR] (STJ11102015)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of human kidney using Aquaporin-1 (AQP1) Rabbit polyclonal antibody (STJ117224) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AQP1 antibody (200-269) (STJ117224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry of rat kidney with Anti-AQP1 primary antibody (STJ500114) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-AQP1 antibody (215-265) (STJ500114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Bovine/Human/Mouse/Rat/Sheep

£427.50

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