• Immunofluorescence analysis of HeLa cells using [KO Validated] Annexin A5/Annexin V Rabbit polyclonal antibody (STJ22620) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from normal (control) and Annexin A5/Annexin V knockout (KO) 293T cells, using Annexin A5/Annexin V antibody (STJ22620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using Annexin A5/Annexin V antibody (STJ22620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ANXA5 antibody (1-320) (STJ22620)

SKU:
STJ22620

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANXA5 (1-320) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANXA5
Gene ID: 308
Uniprot ID: ANXA5_HUMAN
Immunogen Region: 1-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human Annexin A5/Annexin V (NP_001145.1).
Immunogen Sequence: MAQVLRGTVTDFPGFDERAD AETLRKAMKGLGTDEESILT LLTSRSNAQRQEISAAFKTL FGRDLLDDLKSELTGKFEKL IVALMKPSRLYDAYELKHAL KGAGTNEKVLTEIIASRTPE ELRAIKQVYEEEYGSSLEDD VVGDTSGYYQRMLVVLLQAN RDPDAGIDEAQVEQDAQALF QAGELKWGTDEEKFITIFGT RSVSHLRKVFDKYMTISGFQ IEETIDRETSGNLEQLLLA
Post Translational Modifications S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Protein Name Annexin A5
Anchorin Cii
Annexin V
Annexin-5
Calphobindin I
Cpb-I
Endonexin Ii
Lipocortin V
Placental Anticoagulant Protein 4
Pp4
Placental Anticoagulant Protein I
Pap-I
Thromboplastin Inhibitor
Vascular Anticoagulant-Alpha
Vac-Alpha
Database Links Reactome: R-HSA-114608
Alternative Antibody Names Anti-Annexin A5 antibody
Anti-Anchorin Cii antibody
Anti-Annexin V antibody
Anti-Annexin-5 antibody
Anti-Calphobindin I antibody
Anti-Cpb-I antibody
Anti-Endonexin Ii antibody
Anti-Lipocortin V antibody
Anti-Placental Anticoagulant Protein 4 antibody
Anti-Pp4 antibody
Anti-Placental Anticoagulant Protein I antibody
Anti-Pap-I antibody
Anti-Thromboplastin Inhibitor antibody
Anti-Vascular Anticoagulant-Alpha antibody
Anti-Vac-Alpha antibody
Anti-ANXA5 antibody
Anti-ANX5 antibody
Anti-ENX2 antibody
Anti-PP4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance