Primary Antibodies | St John's Laboratory

Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HDAC6 (ABT-HDAC6) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 131kDaObserved band size: 130, 180kDa

Anti-HDAC6 antibody (1100-1215 aa) [ABT-HDAC6] (STJ196790)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse

£180.00 - £261.00

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LnCap whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-PSA (ABT-PSA) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: LnCapPredicted band size: 28kDaObserved band size: 28kDa

Anti-KLK3 antibody (100-200 aa) [ABT-PSA] (STJ196776)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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A431 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK14 (ABT049) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A431Predicted band size: 53kDaObserved band size: 53kDa

Anti-KRT14 antibody (400-472 aa) [PT1674] (STJ196754)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human tonsil, primary antibody was diluted at 1:200. Secondary STJ196750

Anti-CD68 antibody (250-354 aa) [ABT-CD68] (STJ196750)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£180.00 - £261.00

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Western Blot (WB): Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Annexin A1 STJ196742

Anti-ANXA1 antibody (250-346 aa) [ABT-ANXA1] (STJ196742)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£0.00

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Human appendix tissue was stained with Anti-Claudin 7 (ABT443) Antibody

Anti-CLDN7 antibody (100-211 aa) [ABT443] (STJ196726)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Immunohistochemistry - Paraffin (IHC-P): Human parathyroidoma tissue was stained with Anti-Chromogranin A (ABT037) STJ196723

Anti-CHGA antibody (19-94 aa) [ABT037] (STJ196723)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemical analysis of paraffin-embedded Human BrainTissue using HSC70 mouse Monoclonal antibody diluted at 1:200.

Anti-HSPA8 antibody [7G12] (STJ196659)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Human appendix tissue was stained with anti-CD2 (ABT529) antibody.

Anti-CD2 antibody (1-100 aa) [ABT529] (STJ190019)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£180.00 - £261.00

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IHC staining mouse tumor tissue using Anti-Vimentin antibody green with E-cadherin co-stain STJA0003823

Anti-Vimentin antibody (STJA0003823)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole

£438.50

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Western blot 24 kDa UCHL1 protein immunolabeling in rat hippocampal homogenate STJA0003820

Anti-UCHL1 antibody (Full-Length) [BH7] (STJA0003820)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Pig/Rat/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole

£488.50

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IHC staining RBPMS in mouse retinal ganglion cells shown in red STJA0003764

Anti-RBPMS antibody (N-Term) (STJA0003764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Feline/Guinea Pig/Pig/Human/Mouse/Non-Human Primates/Rabbit/Rat/Tree Shrew/Whale/Zebrafish/Canine/Hamster

£488.50

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ICC staining rat cortical neuroepithelial cells using anti-Olig2 antibody red fluorescence STJA0003732

Anti-OLIG2 antibody (STJA0003732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC/IP

Reactivity :

Human/Mouse/Rat

£488.50

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Western blot 200 kDa NF-H protein immunolabeling in rat cortical lysate STJA0003698

Anti-NEFH antibody (STJA0003698)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

All Mammalian/Chicken

£438.50

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MeCP2 antibody IHC staining in rat cerebellum section neuronal nuclei in red STJA0003679

Anti-MECP2 antibody (Full-Length) [5H12] (STJA0003679)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

£488.50

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Anti-GFAP IHC staining astroglial network in mouse hippocampus section STJA0003659

Anti-GFAP antibody (STJA0003659)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

£438.50

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Anti-GABRA1 antibody (Cytoplasmic Loop) (STJA0003639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Mouse/Rat/Bovine/Canine/Human/Non-Human Primates

£488.50

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IHC staining with phospho-ERK-MAPK in mouse hippocampus dentate gyrus granule cells STJA0003631

Anti-Phospho-MAPK1/2-Thr202/Tyr204 antibody (STJA0003631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates/Xenopus/Zebrafish

£488.50

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IHC staining mouse piriform cortex using Anti-Phospho-Ser133 CREB antibody with DAPI counterstain STJA0003605

Anti-Phospho-CREB1-Ser133 antibody (STJA0003605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Sheep/Xenopus/Zebrafish

£488.50

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Western blot analysis of lysates from HeLa cells, using Phospho-ATR-T1989 Rabbit pAb (STJ11103765) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATR-T1989 antibody (STJ11103765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from PC-12 cells, using Phospho-PTEN-S380/T382/383 Rabbit pAb (STJ11103758) at 1:1000 dilution. PC-12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PTEN-S380/T382/S383 antibody (STJ11103758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from K-562 cells, using Phospho-FOXO3A-S425 Rabbit pAb (STJ11103738) at 1:1000 dilution. K-562 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-FOXO3A-S425 antibody (STJ11103738)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from 293T cells, using Phospho-c-Jun-S63 Rabbit pAb (STJ11103730) at 1:1000 dilution. 293T cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-cJun-S63 antibody (STJ11103730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Rabbit anti His-Tag pAb (STJ11103707) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-His-Tag antibody (STJ11103707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP

Reactivity :

Species Independent

£156.50 - £290.50

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Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Rabbit anti Myc-Tag pAb (STJ11103699) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Myc-Tag antibody (C-Term) (STJ11103699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP

Reactivity :

Species Independent

£132.50 - £241.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with DDDDK-Tag using Rabbit anti DDDDK-Tag pAb (STJ11103696) at 1:10000 dilution. <br/>Secondary antibody: (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-DDDDK-Tag antibody (STJ11103696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP

Reactivity :

Species Independent

£156.50 - £290.50

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (STJ11103690) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) (STJ11103690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£201.50 - £379.50

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Western blot analysis of various lysates using Alpha-Tubulin Rabbit pAb (STJ11103685) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-Tubulin antibody (1-180) (STJ11103685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£156.50 - £290.50

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Western blot analysis of various lysates using KIF4A Rabbit mAb (STJ11103648) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-KIF4A antibody (1-100) [S8MR] (STJ11103648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from HepG2 cells, using RBP4 Rabbit mAb (STJ11103635) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-RBP4 antibody (102-201) [S5MR] (STJ11103635)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of various lysates using BAG2 Rabbit mAb (STJ11103631) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-BAG2 antibody (1-100) [S1MR] (STJ11103631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of lysates from Mouse large intestine, using Cytokeratin 16 (KRT16) Rabbit mAb (STJ11103629) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Cytokeratin 16 antibody (1-100) [S9MR] (STJ11103629)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates using TROP-2 Rabbit pAb (STJ11103619) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1s.

Anti-TROP-2 antibody (27-270) (STJ11103619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Confocal imaging of paraffin-embedded Human placenta using CYP26A1 Rabbit monoclonal antibody (STJ11103603, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-CYP26A1 antibody (201-450) [S3MR] (STJ11103603)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded human placenta using ERAP1 Rabbit monoclonal antibody (STJ11103602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-ERAP1 antibody (842-941) [S2MR] (STJ11103602)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of mouse large intestine cells using CEACAM6 Rabbit monoclonal antibody (STJ11103601) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CEACAM6 antibody (200-300) [S1MR] (STJ11103601)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Confocal imaging of HeLa cells using p53 DINP1 Rabbit monoclonal antibody (STJ11103596, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-p53 DINP1 antibody (100-200) [S6MR] (STJ11103596)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of NIH-3T3 cells using WAPL Rabbit monoclonal antibody (STJ11103590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-WAPL antibody (1-100) [S0MR] (STJ11103590)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded rat testis using NELFCD Rabbit polyclonal antibody (STJ11103577) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NELFCD antibody (40-182) (STJ11103577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Confocal imaging of paraffin-embedded Rat skin using Cytokeratin 10 Rabbit monoclonal antibody (STJ11103574, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Cytokeratin 10 antibody (485-584) [S4MR] (STJ11103574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Confocal imaging of C2C12 cells using PMP70/ABCD3 Rabbit monoclonal antibody (STJ11103558, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PMP70/ABCD3 antibody (560-659) [S8MR] (STJ11103558)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of HeLa cells using BANF1 Rabbit monoclonal antibody (STJ11103533) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BANF1 antibody (1-89) [S3MR] (STJ11103533)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of U-2 OS cells using LIS1 Rabbit monoclonal antibody (STJ11103530) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-LIS1 antibody (1-100) [S0MR] (STJ11103530)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of paraffin-embedded mouse liver using ADAMTS13 Rabbit monoclonal antibody (STJ11103516) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ADAMTS13 antibody (50-150) [S6MR] (STJ11103516)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of HeLa cells, using Gli3 antibody (STJ11103514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Gli3 antibody (1301-1580) [S4MR] (STJ11103514)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Confocal imaging of paraffin-embedded Human thyroid using NKX2-1 Rabbit monoclonal antibody (STJ11103513, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.

Anti-TTF1 antibody (1-100) [S3MR] (STJ11103513)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of VCP in paraffin-embedded mouse stomach using VCP Rabbit polyclonal antibody (STJ11103509) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-VCP antibody (460-770) (STJ11103509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of p73 in paraffin-embedded human cervix cancer tissue using p73 Rabbit monoclonal antibody (STJ11103501) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-p73 antibody (1-100) [S1MR] (STJ11103501)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

Choose Options