• Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (STJA0003605, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).
  • Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 800 units/1mg protein for 30 min).
  • Immunolabeling of mouse cerebral cortex (top) and habenula (bottom) brain sections labeled with anti-phospho-ser133 CREB (STJA0003605, DAB, 1:100). These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).
  • Immunolabeling of mouse hippocampal section labeled with anti-phospho-ser133 CREB (STJA0003605, DAB, 1:100, top). The image on the bottom is a magnification of the dentate gyrus section of the hippocampus. These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).

Anti-Phospho-CREB1-Ser133 antibody (STJA0003605)

SKU:
STJA0003605-100

£442.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Sheep/Xenopus/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-CREB-Ser133 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC 1:100-1:1000
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Creb1
Gene ID: 81646
Uniprot ID: CREB1_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH.
Post Translational Modifications Phosphorylation of Ser-119 allows CREBBP binding. Stimulated by phosphorylation. Phosphorylation of both Ser-128 and Ser-119 in the SCN regulates the activity of CREB and participate in circadian rhythm generation. Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-119. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-128. Phosphorylation of Ser-128 blocks CREB-mediated transcription even when Ser-119 is phosphorylated. Phosphorylated by CaMK1. Phosphorylation of Ser-257 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-119 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. CREBL2 positively regulates phosphorylation at Ser-119 thereby stimulating CREB1 transcriptional activity. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion. Phosphorylated by TSSK4 on Ser-119. Sumoylated with SUMO1. Sumoylation on Lys-290, but not on Lys-271, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
Function Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling.
Protein Name Cyclic Amp-Responsive Element-Binding Protein 1
Creb-1
Camp-Responsive Element-Binding Protein 1
Database Links Reactome: R-RNO-198693
Reactome: -RNO-199920
Reactome: -RNO-375165
Reactome: -RNO-442742
Reactome: -RNO-881907
Reactome: -RNO-9031628
Reactome: -RNO-9634638
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cyclic Amp-Responsive Element-Binding Protein 1 antibody
Anti-Creb-1 antibody
Anti-Camp-Responsive Element-Binding Protein 1 antibody
Anti-Creb1 antibody
Anti-Creb-1 antibody

Information sourced from Uniprot.org

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