| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-BANF1 (1-89) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S3MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BANF1 |
| Gene ID: |
8815 |
| Uniprot ID: |
BAF_HUMAN |
| Immunogen Region: |
1-89 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-89 of human BANF1 (O75531). |
| Immunogen Sequence: |
MTTSQKHRDFVAEPMGEKPV GSLAGIGEVLGKKLEERGFD KAYVVLGQFLVLKKDEDLFR EWLKDTCGANAKQSRDCFGC LREWCDAFL |
| Tissue Specificity | Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes. |
| Post Translational Modifications | Ser-4 is the major site of phosphorylation as compared to Thr-2 and Thr-3. Phosphorylation on Thr-2.Thr-3 and Ser-4 disrupts its ability to bind DNA and reduces its ability to bind LEM domain-containing proteins. Non phosphorylated BAF seems to enhance binding between EMD and LMNA. Dephosphorylated by protein phosphatase 2A (PP2A) following interaction with ANKLE2/LEM4 during mitotic exit, leading to mitotic nuclear envelope reassembly. (Microbial infection) Phosphorylated by poxvirus B1 kinase (VPK1) on serine and threonine residues, leading to BANF1 relocalization to the cytoplasm, loss of dimerization and impaired DNA binding activity. (Microbial infection) Phosphorylated at the N-terminus by vaccinia virus (VacV) B1 kinase, leading to BANF1 relocalization to the cytoplasm, loss of dimerization and impaired DNA binding activity. Hyperphosphorylation is linked to the loss of ability to suppress vaccinia virus replication. |
| Function | Non-specific DNA-binding protein that plays key roles in mitotic nuclear reassembly, chromatin organization, DNA damage response, gene expression and intrinsic immunity against foreign DNA. Contains two non-specific double-stranded DNA (dsDNA)-binding sites which promote DNA cross-bridging. Plays a key role in nuclear membrane reformation at the end of mitosis by driving formation of a single nucleus in a spindle-independent manner. Transiently cross-bridges anaphase chromosomes via its ability to bridge distant DNA sites, leading to the formation of a dense chromatin network at the chromosome ensemble surface that limits membranes to the surface. Also acts as a negative regulator of innate immune activation by restricting CGAS activity toward self-DNA upon acute loss of nuclear membrane integrity. Outcompetes CGAS for DNA-binding, thereby preventing CGAS activation and subsequent damaging autoinflammatory responses. Also involved in DNA damage response: interacts with PARP1 in response to oxidative stress, thereby inhibiting the ADP-ribosyltransferase activity of PARP1. Involved in the recognition of exogenous dsDNA in the cytosol: associates with exogenous dsDNA immediately after its appearance in the cytosol at endosome breakdown and is required to avoid autophagy. In case of poxvirus infection, has an antiviral activity by blocking viral DNA replication. (Microbial infection) Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. |
| Protein Name | Barrier-To-Autointegration FactorBreakpoint Cluster Region Protein 1 Cleaved Into - Barrier-To-Autointegration Factor - N-Terminally Processed |
| Database Links | Reactome: R-HSA-162592Reactome: R-HSA-164843Reactome: R-HSA-175567Reactome: R-HSA-177539Reactome: R-HSA-180689Reactome: R-HSA-180910Reactome: R-HSA-2980766Reactome: R-HSA-2995383 |
| Cellular Localisation | NucleusChromosomeNucleus EnvelopeCytoplasmSignificantly Enriched At The Nuclear Inner MembraneDiffusely Throughout The Nucleus During Interphase And Concentrated At The Chromosomes During The M-PhaseThe Phosphorylated Form (By Vrk1) Shows A Cytoplasmic Localization Whereas The Unphosphorylated Form Locates Almost Exclusively In The NucleusMay Be Included In Hiv-1 Virions Via Its Interaction With Viral Gag Polyprotein |
| Alternative Antibody Names | Anti-Barrier-To-Autointegration Factor antibodyAnti-Breakpoint Cluster Region Protein 1 Cleaved Into - Barrier-To-Autointegration Factor - N-Terminally Processed antibodyAnti-BANF1 antibodyAnti-BAF antibodyAnti-BCRG1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
