• Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Myc-Tag antibody (STJ11103699) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of HeLa-Myc-CandHeLa cells using rabbit anti Myc-Tag polyclonal antibody (STJ11103699) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g rabbit anti Myc-Tag polyclonal antibody antibody (STJ11103699). Western blot was performed from the immunoprecipitate using rabbit anti Myc-Tag polyclonal antibody antibody (STJ11103699) at a dilution of 1:50000.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T-NLK-Myc cells using 3 Mu g Myc-Tag antibody (STJ11103699). Western blot was performed from the immunoprecipitate using Myc-Tag antibody (STJ11103699) at a dilution of 1:20000.

Anti-Myc-Tag antibody (C-Term) (STJ11103699)

SKU:
STJ11103699

£140.50 - £212.50
Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Myc-Tag (C-Term) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:10000
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to Myc tag.
Immunogen Sequence: EQKLISEEDL
Background This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance