• Western blot analysis of lysates from wild type (WT) and 293T cells transfected with rabbit anti DDDDK-Tag polyclonal antibody, using rabbit anti DDDDK-Tag polyclonal antibody (STJ11103696) at 1:40000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
  • Immunofluorescence analysis of 293T cells transfected with Flag-c (mCherry) and 293T cells transfected with Flag-N (mCherry) use rabbit anti DDDDK-Tag polyclonal antibody (STJ11103696) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of 293T cells transfected with Flag-N (mCherry) and untreated 293T cells use rabbit anti DDDDK-Tag polyclonal antibody (STJ11103696) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T-SERPINB1-Flag-GFP cells using 3 Mu g DDDDK-Tag polyclonal antibody (STJ11103696). Western blot was performed from the immunoprecipitate using DDDDK-Tag polyclonal antibody (STJ11103696) at a dilition of 1:50000.
  • Anti-DDDDK-Tag antibody (STJ11103696)

Anti-DDDDK-Tag antibody (STJ11103696)

SKU:
STJ11103696-100

£166.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDDDK-Tag is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic peptide corresponding to DDDDK tag.
Immunogen Sequence: DYKDDDDK
Background The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

Information sourced from Uniprot.org

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