St John's Laboratory Ltd

STJ70401 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-FBL2/FBXL2 antibody (N-Term) (STJ70401)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF

Reactivity :

Human/Rat/Dog/Cow

£312.50

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Anti-SPOP antibody (325-374) {FITC} (STJ506214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Human VHL protein (Recombinant) (N-His) (STJP007981)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-Fbxw7 antibody (STJA0037917)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£187.50 - £281.50

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Human SUMO3 protein (Recombinant) (N-His) (STJP014544)

Host :

E.coli

Applications :

ELISA/WB

£319.50

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Western blot analysis of various lysates using FAU Rabbit polyclonal antibody (STJ114074) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FAU antibody (1-133) (STJ114074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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SPOP Positive Control for STJ506213 peptide (STJ505903)

Applications :

WB

£308.50

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STJ70347 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-TRIM11 antibody (C-Term) (STJ70347)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

£312.50

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SPOP Blocking Peptide for STJ506214 peptide (STJ505902)

Applications :

Immunodepletion/Immunocompetition

£260.50

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Anti-SPOP antibody (325-374) {Biotin} (STJ506213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C overnight STJ195080

Anti-KLHL2 antibody (521-571 aa) (STJ195080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Human UBA2/SAE2 protein (Recombinant) (N-His) (STJP009398)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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STJ72723 (0. 3µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-TRIM11 antibody (249-263) (STJ72723)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Pig/Cow

£312.50

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Anti-SPOP antibody (325-374) (STJ506212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-TRIM11 antibody (100-150 aa) (STJ13102529)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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TRIM21 antibody western blot K562 HeLa STJA0034951

Anti-TRIM21 antibody [R01-1E8] (STJA0034951)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Biotinylated STJ70294 (0. 5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-UBE2I, Biotinylated antibody (N-Term) {Biotin} (STJ73262)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Anti-UBE2I antibody (STJA0010235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-TIF1/TRIM24 IP antibody [SAA0265] (STJA0018780)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£195.50 - £1,319.50

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SDS-PAGE gel of recombinant mouse Elongin B protein 3ug under reducing conditions Coomassie blue stain

Mouse Elongin B protein (Recombinant) (His-Tag) (STJP019394)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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Human SUMO3 protein (Recombinant) (C-His) (STJP013380)

Host :

Mammalian Cells

Applications :

ELISA/WB

£454.50

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RNF20 antibody western blot MCF-7 HeLa lysates STJA0035763

Anti-RNF20 antibody [R07-7H7] (STJA0035763)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human/Mouse

£133.50 - £281.50

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Immunohistochemistry analysis of paraffin-embedded mouse testis using KLHL9 antibody (STJ112188) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-KLHL9 antibody (1-100) (STJ112188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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SDS-PAGE gel of recombinant human SPSB2 protein 3µg under reducing conditions stained with Coomassie blue

Human SPSB2 protein (Recombinant) (His-Tag) (STJP017888)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £285.50

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TRIM21 antibody western blot MCF-7 293T HeLa STJA0032505

Anti-TRIM21 antibody [5B9-B9-H8] (STJA0032505)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MAGEF1 antibody (30-110 aa) (STJ190365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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SDS-PAGE of recombinant human Tollip protein 3µg under reducing conditions Coomassie blue stain

Human Tollip protein (Recombinant) (His-Tag) (STJP017202)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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Anti-Recombinant-TRAF6 antibody [RM2L14] (STJA0025978)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£300.50 - £457.50

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SDS-PAGE gel of recombinant human UBA5 protein 3µg under reducing conditions with Coomassie blue staining

Human UBA5 protein (Recombinant) (His-Tag) (STJP017623)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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Human TRAF6 protein (Recombinant) (N-His) (STJP006268)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Western blot analysis of various cells using TRAF6 Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Anti-TRAF6 antibody (105-154 aa) (STJ96085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Recombinant-TRAF6 antibody [RM1A79] (STJA0026750)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-TRAF6 antibody [PT0707R] (STJA0039174)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various lysates using RNF40 Rabbit mAb (STJ11102500) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-RNF40 antibody (231-431) [S0MR] (STJ11102500)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of various lysates using RNF20 Rabbit polyclonal antibody (STJ119439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RNF20 antibody (1-260) (STJ119439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Human TAX1BP1 protein (Recombinant) (N-His) (STJP007698)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Human SUMO3 protein (Recombinant) (Tag free) (STJP014545)

Host :

E.coli

Applications :

ELISA/WB

£414.50

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SDS-PAGE gel of recombinant human UBL4A protein under reducing conditions stained with Coomassie blue

Human UBL4A protein (Recombinant) (His-Tag) (STJP017541)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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RNF40 antibody western blot HeLa RAW2647 lysates STJA0037039

Anti-RNF40 antibody [R05-9V4] (STJA0037039)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Mouse/Rat/Human

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-VHL-Ser68 antibody (34-83 aa) (STJ91273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200

Anti-VHL antibody (1-50 aa) (STJ98809)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Human UBE2V2 protein (Recombinant) (N-His) (STJP014642)

Host :

E.coli

Applications :

ELISA/WB

£503.50

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Western blot analysis of extracts of various cell lines, using RNF40 antibody (STJ28526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RNF40 antibody (1-300) (STJ28526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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STJ70329 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-GERP/TRIM8 antibody (C-Term) (STJ70329)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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Western blot analysis of extracts of various cell lines, using TRIM21/SS-A antibody (STJ115508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TRIM21 antibody (86-334) (STJ115508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with STJ70342 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with STJ70342 (1mg/ml) in Lane B. Detected by chemiluminescence.

Anti-SSA1/Ro52 antibody (C-Term) (STJ70342)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB-Trf/IF/FC

Reactivity :

Human

£312.50

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Immunofluorescence analysis of L929 cells using VHL Rabbit polyclonal antibody (STJ118719) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VHL antibody (1-100) (STJ118719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using TRIM41 Rabbit polyclonal antibody (STJ26386) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-TRIM41 antibody (421-630) (STJ26386)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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