Anti-VHL antibody (167-186) (STJ26089)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal VHL (167-186) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VHL |
| Gene ID | 7428 |
| Uniprot ID | VHL_HUMAN |
| Immunogen Region | 167-186 |
| Immunogen Sequence | VWLNFDGEPQPYPTLPPGTG RRIHSYRGHLWLFRDAGTHD GLLVNQTELFVPSLNVDGQP IFANITLPVYTLKERCLQVV RSLVKPENYRRLDIVRSLYE DLEDHPNVQKDLERLTQERI AHQRMGD |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 87-186 of human VHL (NP_000542.1). |
Additional Info
| Tissue Specificity | Expressed in the adult and fetal brain and kidney. |
| Function | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR. |
| Protein Name | Von Hippel-Lindau Disease Tumor SuppressorProtein G7Pvhl |
| Database Links | Reactome: R-HSA-1234176Reactome: R-HSA-3232142Reactome: R-HSA-8951664Reactome: R-HSA-9682706Reactome: R-HSA-9694686Reactome: R-HSA-9706019Reactome: R-HSA-983168 |
| Cellular Localisation | Isoform 1: CytoplasmCell MembranePeripheral Membrane ProteinEndoplasmic ReticulumNucleusFound Predominantly In The Cytoplasm And With Less Amounts Nuclear Or Membrane-AssociatedColocalizes With Adrb2 At The Cell MembraneIsoform 3: CytoplasmEqually Distributed Between The Nucleus And The Cytoplasm But Not Membrane-Associated |
| Alternative Antibody Names | Anti-Von Hippel-Lindau Disease Tumor Suppressor antibodyAnti-Protein G7 antibodyAnti-Pvhl antibodyAnti-VHL antibody |
Information sourced from Uniprot.org