| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-UBASH3B (1-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UBASH3B |
| Gene ID: |
84959 |
| Uniprot ID: |
UBS3B_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human UBASH3B (NP_116262.2). |
| Immunogen Sequence: |
MAQYGHPSPLGMAAREELYS KVTPRRNRQQRPGTIKHGSA LDVLLSMGFPRARAQKALAS TGGRSVQAACDWLFSHVGDP FLDDPLPREYVLYLRPTGPL AQKLSDFWQQSKQICGKNKA HNIFPHITLCQFFMCEDSKV DALGEALQTTVSRWKCKFSA PLPLELYTSSNFIGLFVKED SAEVLKKFAADFAAEAASKT EVHVEPHKKQLHVTLAYHFQ ASHLPTLEKLAQNIDVKLG |
| Function | Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination. |
| Protein Name | Ubiquitin-Associated And Sh3 Domain-Containing Protein BCbl-Interacting Protein P70Suppressor Of T-Cell Receptor Signaling 1Sts-1T-Cell Ubiquitin Ligand 2Tula-2Tyrosine-Protein Phosphatase Sts1/Tula2 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Ubiquitin-Associated And Sh3 Domain-Containing Protein B antibodyAnti-Cbl-Interacting Protein P70 antibodyAnti-Suppressor Of T-Cell Receptor Signaling 1 antibodyAnti-Sts-1 antibodyAnti-T-Cell Ubiquitin Ligand 2 antibodyAnti-Tula-2 antibodyAnti-Tyrosine-Protein Phosphatase Sts1/Tula2 antibodyAnti-UBASH3B antibodyAnti-KIAA1959 antibodyAnti-STS1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
