Anti-MAGEF1 antibody (30-110 aa) (STJ190365)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 30-110
General Information
| Short Description | Rabbit polyclonal anti-Melanoma-associated antigen F1 (30-110 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAGEF1 |
| Gene ID | 64110 |
| Uniprot ID | MAGF1_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 30-110 |
| Immunogen Region | 30-110 aa |
| Specificity | MAGF1 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Function | Enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin ligases. Proposed to act through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. MAGEF1-NSMCE1 ubiquitin ligase complex promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. May negatively regulate genome integrity by inhibiting homologous recombination-mediated double-strand break DNA repair. |
| Protein Name | Melanoma-Associated Antigen F1Mage-F1Mage-F1 Antigen |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Melanoma-Associated Antigen F1 antibodyAnti-Mage-F1 antibodyAnti-Mage-F1 Antigen antibodyAnti-MAGEF1 antibody |
Information sourced from Uniprot.org