St John's Laboratory Ltd

Human SH3PXD2A protein (Recombinant) (N-His) (STJP007695)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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PRPF19 antibody western blot HepG2 NIH/3T3 STJA0036035

Anti-PRPF19 antibody [R04-2I5] (STJA0036035)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of lysates from HeLa cells, using SH3PXD2A Rabbit pAb (STJ11102477). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SH3PXD2A antibody (806-1105) (STJ11102477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PPP1R12A-Ser507 antibody (STJ196361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using UIMC1 Rabbit polyclonal antibody (STJ29387). Green：GFP-RNF168 fusion protein expression for DNA damage marker. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Anti-UIMC1 antibody (1-150) (STJ29387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of A549 cells, using MYPT1 (Phospho-Thr696) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PPP1R12A-Thr696 antibody (661-710 aa) (STJ91291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-RAP80 antibody (Internal) (STJ71994)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig

£312.50

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Human PPP1R12A protein (Recombinant) (N-His) (STJP009576)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-NCK1 antibody [EM-06] (STJ16103375)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Mouse/Human

£560.50

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PPP1R12A-Ser668 antibody (STJ196362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-MYPT1 antibody [PT0206R] (STJA0039424)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human

£285.00 - £540.00

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Anti-CEP164 antibody (STJA0006757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£49.00 - £261.00

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Anti-UIMC1 antibody (STJ191585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NCK1 antibody (STJ190734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Recombinant-UIMC1 antibody [RM2T05] (STJA0026662)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human

£300.50 - £457.50

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Human NCK1 protein (Recombinant) (N-His) (STJP004164)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Human NCK1 protein (Recombinant) (N-His) (STJP014426)

Host :

E.coli

Applications :

ELISA/WB

£503.50

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Immunofluorescence analysis of human-lung tissue. 1, MYPT1 (phospho Thr853) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-PPP1R12A-Thr853 antibody (621-670 aa) (STJ90822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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RPA70 antibody western blot HeLa A431 MCF-7 COS7 HT-1080 K562 STJA0012829

Anti-RPA1 antibody (317-616 aa) [8C3-D12-H10] (STJA0012829)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Monkey/Mouse/Rat

£133.50 - £281.50

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RPA70 antibody western blot A549 lysates STJA0034822

Anti-RPA70 antibody [R04-7B7] (STJA0034822)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from mouse brain, using NCK1 Rabbit polyclonal antibody (STJ24702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NCK1 antibody (58-377) (STJ24702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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NCK1 antibody immunocytochemistry HeLa cells STJA0034609

Anti-NCK1 antibody [R09-7F6] (STJA0034609)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PPP1R12A-Ser668/Ser618 antibody (STJA0010031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using SLFN11 antibody (STJ11103342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SLFN11 antibody (132-328) (STJ11103342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot FANCI protein phosphorylated at Ser559 in HeLa cell lysate STJA0003831

Anti-Phospho-FANCI-Ser559 antibody (STJA0003831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

£488.50

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Anti-Mouse-H+L nanobody {AbFluor 594} (STJN000658)

Clonality :

Monoclonal

Host :

Camel VHH

Applications :

mIHC/IF

Reactivity :

Mouse

£64.50 - £329.50

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Anti-CEP164 nanobody [36Z] (STJN000702)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£195.50 - £1,319.50

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Western blot analysis of extracts of various cell lines, using RPA70/RPA1 rabbit monoclonal antibody (STJ11101855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RPA1 antibody (1-100) [S5MR] (STJ11101855)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP

Reactivity :

Human/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of RPA70/RPA1 in paraffin-embedded human uterus using RPA70/RPA1 Rabbit polyclonal antibody (STJ114904) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-RPA1 antibody (1-240) (STJ114904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-RPA1 antibody (STJA0010246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Monkey/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ATAD5 antibody (1054-1104 aa) (STJ195862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Human EXO1 protein (Recombinant) (N-His) (STJP006330)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Western blot analysis of various lysates using EPS15 Rabbit pAb (STJ111856) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-EPS15 antibody (660-840) (STJ111856)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Recombinant-NCK1 antibody [RM1Q07] (STJA0023991)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g PPP1R12A antibody (STJ113547). Western blot was performed from the immunoprecipitate using PPP1R12A antibody (STJ113547) at a dilution of 1:1000.

Anti-PPP1R12A antibody (1-200) (STJ113547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse heart using PPP1R12A Antibody (STJ28783) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PPP1R12A antibody (710-970) (STJ28783)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYPT1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PPP1R12A antibody (621-670) (STJ94320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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STJ71446 (0. 2µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-EXO1 antibody (Internal) (STJ71446)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of Lamin A/C at dilution of 1:2000

Anti-Lamin A + Lamin C antibody [SM5079] (STJA0005079)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Western blot analysis of various lysates using Phospho-PPP1R12A-S668 Rabbit pAb (STJ11100976) at 1:2000 dilution. Both HeLa cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PPP1R12A-S668 antibody (STJ11100976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using NCK11 Rabbit mAb (STJ11102430) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-NCK1 antibody (200-300) [S0MR] (STJ11102430)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates using Phospho-PPP1R12A-S507 Rabbit pAb (STJ116389) at 1:2000 dilution or PPP1R12A antibody (STJ113547). HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PPP1R12A-S507 antibody (STJ116389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of NIH-3T3 cells using Cleaved-Lamin A (N231) Polyclonal Antibody

Anti-Cleaved-LMNA-N231 antibody (212-261 aa) (STJ90057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Lamin A/C Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Galectin-3 monoclonal antibody (6G2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-LMNA antibody (361-410 aa) (STJ93885)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Mouse LMNA (Lamin A/C) Sandwich ELISA Kit (STJE0010366)

Applications :

ELISA

Reactivity :

Mouse

£432.50 - £601.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Lamin A/C (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-LMNA-Ser392 antibody (361-410 aa) (STJ91042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using MCM2 antibody (STJ24523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MCM2 antibody (360-700) (STJ24523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-Recombinant-TIMELESS antibody [RM2RM07] (STJA0026466)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human

£300.50 - £457.50

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