St John's Laboratory Ltd

Western blot analysis of extracts of various cell lines, using FANCI antibody (STJ23625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FANCI antibody (1061-1328) (STJ23625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SH3PXD2A antibody (N-Term) (STJ93078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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western blot FANCI protein phosphorylated at Ser556 in HeLa cell lysate STJA0003633

Anti-Phospho-FANCI-Ser556 antibody (STJA0003633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Goat/Non-Human Primates/Sheep

£488.50

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Immunofluorescence analysis of MCF7 cell. 1, primary Antibody was diluted at 1:100 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody (catalog No: STJS000476) was diluted at 1:500 (room temperature, 50min).

Anti-Phospho-USP28-Ser67 antibody (STJA0009968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-CEP164 antibody (860-920 aa) (STJ13100993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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USP28 antibody western blot SW480 lysates STJA0037211

Anti-USP28 antibody [R03-8X4] (STJA0037211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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USP28 antibody western blot A549 cells STJA0034983

Anti-USP28 antibody [R08-7I7] (STJA0034983)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PPP1R12A-Ser668 antibody (STJ196362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of JK cells using Phospho-Eps15 (Y849) Polyclonal Antibody

Anti-Phospho-EPS15-Tyr849 antibody (STJ90629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-VCPIP1 antibody (STJA0008148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Human NCK1 protein (Recombinant) (N-His) (STJP004164)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NCK1 antibody (STJ190734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Recombinant-UIMC1 antibody [RM2T05] (STJA0026662)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human

£300.50 - £457.50

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Anti-MYPT1 antibody [PT0206R] (STJA0039424)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human

£285.00 - £540.00

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Human NCK1 protein (Recombinant) (N-His) (STJP014426)

Host :

E.coli

Applications :

ELISA/WB

£503.50

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Western blot analysis of various lysates using EPS15 Rabbit pAb (STJ111856) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-EPS15 antibody (660-840) (STJ111856)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Recombinant-NCK1 antibody [RM1Q07] (STJA0023991)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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RAP80 antibody immunocytochemistry HeLa cells STJA0035348

Anti-RAP80 antibody [R01-0D5] (STJA0035348)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using UIMC1 Rabbit polyclonal antibody (STJ29387). Green：GFP-RNF168 fusion protein expression for DNA damage marker. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Anti-UIMC1 antibody (1-150) (STJ29387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of A549 cells, using MYPT1 (Phospho-Thr696) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PPP1R12A-Thr696 antibody (661-710 aa) (STJ91291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-NCK1 antibody [EM-06] (STJ16103375)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Mouse/Human

£560.50

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Western blot analysis of lysates from HeLa cells, using SH3PXD2A Rabbit pAb (STJ11102477). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SH3PXD2A antibody (806-1105) (STJ11102477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-UIMC1 antibody (STJ191585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Human PPP1R12A protein (Recombinant) (N-His) (STJP009576)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PPP1R12A-Ser507 antibody (STJ196361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of human-lung tissue. 1, MYPT1 (phospho Thr853) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-PPP1R12A-Thr853 antibody (621-670 aa) (STJ90822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-RAP80 antibody (Internal) (STJ71994)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig

£312.50

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Western blot analysis of various lysates using Phospho-PPP1R12A-T853 Rabbit pAb ( STJ11101051) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PPP1R12A-T853 antibody (STJ11101051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-ATAD5/FRAG1 antibody (Internal) (STJ71103)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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NCK1 antibody western blot human recombinant protein STJA0032546

Anti-NCK1 antibody [7H2-4C2-1A2] (STJA0032546)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/FC

Reactivity :

Human/Monkey

£133.50 - £281.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g PPP1R12A antibody (STJ113547). Western blot was performed from the immunoprecipitate using PPP1R12A antibody (STJ113547) at a dilution of 1:1000.

Anti-PPP1R12A antibody (1-200) (STJ113547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse heart using PPP1R12A Antibody (STJ28783) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PPP1R12A antibody (710-970) (STJ28783)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ATAD5 antibody (1054-1104 aa) (STJ195862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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STJ71446 (0. 2µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-EXO1 antibody (Internal) (STJ71446)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-RPA1 antibody (STJA0010246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Monkey/Mouse/Rat

£49.00 - £261.00

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RPA70 antibody western blot HEK293 lysates STJA0036621

Anti-RPA70 antibody [R05-7P8] (STJA0036621)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human

£133.50 - £281.50

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RPA70 antibody western blot C6 COS7 K562 HeLa STJA0038223

Anti-RPA70 antibody [8C3-D12-H10] (STJA0038223)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Monkey/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various cells using Exo1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-EXO1 antibody (61-110 aa) (STJ93011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYPT1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PPP1R12A antibody (621-670) (STJ94320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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SDS-PAGE gel of recombinant human NCK1 protein 3μg under reducing conditions stained with Coomassie blue

Human NCK1 protein (Recombinant) (His-Tag) (STJP016727)

Host :

E.coli

Applications :

SDS-PAGE

£125.50 - £649.50

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PPP1R12A-Ser668/Ser618 antibody (STJA0010031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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NCK1 antibody immunocytochemistry HeLa cells STJA0034609

Anti-NCK1 antibody [R09-7F6] (STJA0034609)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates using Phospho-PPP1R12A-S668 Rabbit pAb (STJ11100976) at 1:2000 dilution. Both HeLa cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PPP1R12A-S668 antibody (STJ11100976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Human EXO1 protein (Recombinant) (N-His) (STJP006330)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Western blot analysis of extracts of various cell lines, using RPA70/RPA1 rabbit monoclonal antibody (STJ11101855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RPA1 antibody (1-100) [S5MR] (STJ11101855)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP

Reactivity :

Human/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of RPA70/RPA1 in paraffin-embedded human uterus using RPA70/RPA1 Rabbit polyclonal antibody (STJ114904) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-RPA1 antibody (1-240) (STJ114904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of Lamin A+ Lamin C. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-Recombinant-Lamin A + Lamin C antibody [SM0637] (STJA0010637)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Western blot analysis of various lysates using NCK11 Rabbit mAb (STJ11102430) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-NCK1 antibody (200-300) [S0MR] (STJ11102430)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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