Anti-Phospho-PPP1R12A-Ser507 antibody (STJ196361)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phosho peptide around human MYPT1 (Ser507)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Protein phosphatase 1 regulatory subunit 12A-Ser507 for use in WB and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PPP1R12A |
| Gene ID | 4659 |
| Uniprot ID | MYPT1_HUMAN |
| Immunogen | Synthesized phosho peptide around human MYPT1 (Ser507) |
| Specificity | This antibody detects endogenous levels of Human Mouse Rat MYPT1 (phospho-Ser507) |
Additional Info
| Post Translational Modifications | Phosphorylated by CIT (Rho-associated kinase). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK.may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A. |
| Function | Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity. |
| Protein Name | Protein Phosphatase 1 Regulatory Subunit 12aMyosin Phosphatase-Targeting Subunit 1Myosin Phosphatase Target Subunit 1Protein Phosphatase Myosin-Binding Subunit |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5627117Reactome: R-HSA-5627123 |
| Cellular Localisation | CytoplasmCytoskeletonStress FiberAlso Along Actomyosin Filaments |
| Alternative Antibody Names | Anti-Protein Phosphatase 1 Regulatory Subunit 12a antibodyAnti-Myosin Phosphatase-Targeting Subunit 1 antibodyAnti-Myosin Phosphatase Target Subunit 1 antibodyAnti-Protein Phosphatase Myosin-Binding Subunit antibodyAnti-PPP1R12A antibodyAnti-MBS antibodyAnti-MYPT1 antibody |
Information sourced from Uniprot.org