• Western blot analysis of lysates from NIH/3T3 cells, using MYPT1 (Phospho-Thr853) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 293T AD293 22RV1 HELA cells using Phospho-MYPT1 (T853) Polyclonal Antibody diluted at 1：2000
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MYPT1 (Phospho-Thr853) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of rat-kidney tissue. 1, MYPT1 (phospho Thr853) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of 293T cells using Phospho-MYPT1 (T853) Polyclonal Antibody diluted at 1：2000
  • Immunofluorescence analysis of rat-kidney tissue. 1, MYPT1 (phospho Thr853) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-heart tissue. 1, MYPT1 (phospho Thr853) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-heart tissue. 1, MYPT1 (phospho Thr853) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-lung tissue. 1, MYPT1 (phospho Thr853) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-lung tissue. 1, MYPT1 (phospho Thr853) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-PPP1R12A-Thr853 antibody (621-670 aa) (STJ90822)

SKU:
STJ90822

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Protein phosphatase 1 regulatory subunit 12A-Thr853 (621-670 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1R12A
Gene ID: 4659
Uniprot ID: MYPT1_HUMAN
Immunogen Region: 621-670 aa
Specificity: Phospho-MYPT1 (T853) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MYPT1 around the phosphorylation site of Thr853 at the amino acid range 621-670
Post Translational Modifications Phosphorylated by CIT (Rho-associated kinase). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK.may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A.
Function Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Protein Name Protein Phosphatase 1 Regulatory Subunit 12a
Myosin Phosphatase-Targeting Subunit 1
Myosin Phosphatase Target Subunit 1
Protein Phosphatase Myosin-Binding Subunit
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5627123
Cellular Localisation Cytoplasm
Cytoskeleton
Stress Fiber
Also Along Actomyosin Filaments
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 12a antibody
Anti-Myosin Phosphatase-Targeting Subunit 1 antibody
Anti-Myosin Phosphatase Target Subunit 1 antibody
Anti-Protein Phosphatase Myosin-Binding Subunit antibody
Anti-PPP1R12A antibody
Anti-MBS antibody
Anti-MYPT1 antibody

Information sourced from Uniprot.org

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