Anti-Recombinant-UIMC1 antibody [RM2T05] (STJA0026662)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJA0026662
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General Information

Short DescriptionRabbit monoclonal anti-Recombinant-Brca1-A Complex Subunit Rap80 for use in IF, IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIF/IHC/WB
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDRM2T05
IsotypeIgG
ConjugationUnconjugated
PurificationProtein A/G purified from cell culture supernatant
Dilution RangeIF: 1:50-1:200, IHC: 1:50-1:100, WB: 1:500-1:1000
Formulation0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide
Storage InstructionSuitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months.

Target Information

Gene SymbolUIMC1
Gene ID51720
Uniprot IDUIMC1_HUMAN

Additional Info

Post Translational Modifications Sumoylated. Phosphorylated upon DNA damage by ATM or ATR.
Function Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B.the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
Protein Name Brca1-A Complex Subunit Rap80
Receptor-Associated Protein 80
Retinoid X Receptor-Interacting Protein 110
Ubiquitin Interaction Motif-Containing Protein 1
Database Links Reactome: R-HSA-5689901
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-69473
Cellular Localisation Nucleus
Localizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs)
Alternative Antibody Names Anti-Brca1-A Complex Subunit Rap80 antibody
Anti-Receptor-Associated Protein 80 antibody
Anti-Retinoid X Receptor-Interacting Protein 110 antibody
Anti-Ubiquitin Interaction Motif-Containing Protein 1 antibody
Anti-UIMC1 antibody
Anti-RAP80 antibody
Anti-RXRIP110 antibody

Information sourced from Uniprot.org

Citations

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