Vesicle-Mediated Transport | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using CAPZA1 antibody (STJ22887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CAPZA1 antibody (1-286) (STJ22887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CAPZA1 antibody (STJA0007084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of CAPZA2 in paraffin-embedded Mouse kidney using CAPZA2 Rabbit polyclonal antibody (STJ22888) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CAPZA2 antibody (1-286) (STJ22888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-CAPZA2 antibody (STJA0007512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-CAPZA3 antibody (STJA0004680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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Anti-CAPZA3 antibody (STJA0004681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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Western blot analysis of extracts of various cell lines, using CAPZB antibody (STJ110405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CAPZB antibody (1-272) (STJ110405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CAPZB antibody (STJA0007368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Cation-independent M6PR antibody (2140-2300 aa) [SRM] (STJ11106854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using Cbl Polyclonal Antibody

Anti-CBL antibody (740-789 aa) (STJ92055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T, using CBL antibody (STJ22913) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CBL antibody (850-906) (STJ22913)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CBL antibody (STJ110191) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-CBL antibody (850-950) (STJ110191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CBL antibody (C-Term) [M159] (STJA0005315)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis using Cbl monoclonal antibody against RAJI (1) , RAW264.7 (2) , K562 (3) , SKBR-3 (4) , 3T3-L1 (5) , THP-1 (6) and PC-12 (7) cell lysate.

Anti-CBL antibody [3B12] (STJ97898)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Anti-CD127/IL-7RA antibody [A019D5] {AF488} (STJA0001893)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£152.50 - £407.50

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Anti-CD127/IL-7RA antibody [A019D5] {AF647} (STJA0001894)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£152.50 - £407.50

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HumanperipheralbloodlymphocytesarestainedwithAnti-Human CD127 Monoclonal Antibody (APC Conjugated) andAnti-Human CD3 Monoclonal Antibody (FITC Conjugated)

Anti-CD127/IL-7RA antibody [A019D5] {APC} (STJA0000936)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£152.50 - £390.50

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Anti-CD127/IL-7RA antibody [A019D5] {EF780} (STJA0016431)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £377.50

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Anti-CD127/IL-7RA antibody [A019D5] {EV450} (STJA0016430)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £377.50

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HumanperipheralbloodlymphocytesarestainedwithAnti-Human CD127 Monoclonal Antibody (FITC Conjugated) and Anti-Human CD3 Monoclonal Antibody (APC Conjugated)

Anti-CD127/IL-7RA antibody [A019D5] {FITC} (STJA0000934)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£142.50 - £310.50

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Anti-CD127/IL-7RA antibody [A019D5] {PE/Cyanine5.5} (STJA0004150)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £377.50

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Anti-CD127/IL-7RA antibody [A019D5] {PE/Cyanine5} (STJA0001891)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£152.50 - £407.50

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Human peripheral blood lymphocytes were stained with Anti-Human CD3 Monoclonal Antibody (FITC Conjugated) and Anti-CD127/IL-7RA antibody (STJA0000937)

Anti-CD127/IL-7RA antibody [A019D5] {PE/Cyanine7} (STJA0000937)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £407.50

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Anti-CD127/IL-7RA antibody [A019D5] {PerCP/Cyanine5.5} (STJA0000938)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£176.50 - £437.50

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Human peripheral blood lymphocytes were stained with Anti-CD127/IL-7RA antibody (STJA0000935) and Anti-Human CD3 Monoclonal Antibody (APC Conjugated).

Anti-CD127/IL-7RA antibody [A019D5] {PE} (STJA0000935)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£142.50 - £365.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD163 (ABT-CD163) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: NCM460 Lane 2: K562Predicted band size: 125kDaObserved band size: 125kDa

Anti-CD163 antibody (1-100 aa) [ABT-CD163] (STJ196804)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD63 using CD163 Rabbit polyclonal antibody (STJ11104977) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD163 antibody (42-261) (STJ11104977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Human lymph node stained with anti-CD163 antibody using peroxidase-conjugate and DAB chromogen. Note membrane staining of histiocytes.

Anti-CD163 antibody (43-196aa) [ZM29] (STJ180301)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£164.50 - £601.50

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Western blot analysis of 293T-CD163, using CD163 rabbit polyclonal antibody (STJ110681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD163 antibody (750-1050) (STJ110681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded mouse spleen using CD163 Rabbit polyclonal antibody (STJ11105484) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-CD163 antibody (810-1049) (STJ11105484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC

Reactivity :

Human/Mouse

£112.50 - £524.50

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Confocal imaging of paraffin-embedded Human spleen tissue using CD163 Rabbit monoclonal antibody (STJ11104893, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

Anti-CD163 antibody (861-961) [S4893RM] (STJ11104893)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-CD163 antibody [10D6] (STJ16101698)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,583.50

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Anti-CD163 antibody [GHI/61] {PerCP/Cyanine 5.5} (STJA0016504)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£163.50 - £395.50

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Anti-CD163 antibody [MRQ-26] (STJ16100324)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,318.50 - £1,358.50

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Anti-CD163 antibody [RM3/1] (STJ16100302)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/IF/IP/WB

Reactivity :

Human/Monkey

£724.50

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Western blot analysis of extracts of various cell lines, using CD36/SR-B3 antibody (STJ28356) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD36 antibody (30-439) (STJ28356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CD36/SR-B3 antibody (STJ11101554) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-CD36 antibody (300-400) [S4MR] (STJ11101554)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from U-937 cells using CD36/SR-B3 Rabbit polyclonal antibody (STJ22993) at 1:700 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:90s.

Anti-CD36 antibody (301-400) (STJ22993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of MCF7, 4T1 cells using CD36 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD36 antibody (331-380 aa) (STJ97280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Tilapia

£48.00 - £252.00

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Immunofluorescence analysis of U937 cells using CD36/SR-B3 Rabbit polyclonal antibody (STJ116914) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD36 antibody (50-150) (STJ116914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CD36/SR-B3 antibody (STJ119465) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD36 antibody (50-150) (STJ119465)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CD36 antibody (ECD) (STJ13100091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0. 01M containing Triton X 0. 005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to extracellular domain of human CD36 (Fatty acid translocase) : IgG (STJ13100092) diluted 1: 100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1: 100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Anti-CD36 antibody (ECD) (STJ13100092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-CD36 antibody (ECD) (STJ13100102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Marmoset/Rat/Mouse

£331.50

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Anti-CD36 antibody (ECD) (STJ13100903)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-CD36 antibody (STJ500463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human

£427.50

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Anti-CD36 antibody [185-1G2] (STJ16102561)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Human ErythroLeukemia cell line HEL were stained with Anti-CD36 antibody (STJA0000953) (filled gray histogram). Unstained Human ErythroLeukemia cell line HEL (empty black histogram) were used as control.

Anti-CD36 antibody [5-271] {APC} (STJA0000953)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£142.50 - £331.50

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