• Immunofluorescence analysis of HeLa cells using ARF6 antibody (STJ22670) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ARF6 antibody (STJ22670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-ARF6 antibody (1-175) (STJ22670)

SKU:
STJ22670

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARF6 (1-175) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARF6
Gene ID: 382
Uniprot ID: ARF6_HUMAN
Immunogen Region: 1-175
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human ARF6 (NP_001654.1).
Immunogen Sequence: MGKVLSKIFGNKEMRILMLG LDAAGKTTILYKLKLGQSVT TIPTVGFNVETVTYKNVKFN VWDVGGQDKIRPLWRHYYTG TQGLIFVVDCADRDRIDEAR QELHRIINDREMRDAIILIF ANKQDLPDAMKPHEIQEKLG LTRIRDRNWYVQPSCATSGD GLYEGLTWLTSNYKS
Tissue Specificity Ubiquitous, with higher levels in heart, substantia nigra, and kidney.
Post Translational Modifications GTP-bound form is myristoylated on Lys-3 by NMT1 and NMT2, allowing ARF6 to remain on membranes during the GTPase cycle, thereby promoting its activity. GDP-bound inactive form is demyristoylated on Lys-3 by SIRT2 at early endosomes or endocytic recycling compartment to allow its efficient activation by a guanine exchange factor (GEF) after GDP release.
Function GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1. Required for NTRK1 sorting to the recycling pathway from early endosomes. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Protein Name Adp-Ribosylation Factor 6
Database Links Reactome: R-HSA-8854214
Reactome: R-HSA-8856828
Reactome: R-HSA-8875656
Cellular Localisation Cytoplasm
Cytosol
Cell Membrane
Lipid-Anchor
Endosome Membrane
Recycling Endosome Membrane
Cell Projection
Filopodium Membrane
Ruffle
Cleavage Furrow
Midbody
Midbody Ring
Early Endosome Membrane
Golgi Apparatus
Trans-Golgi Network Membrane
Distributed Uniformly On The Plasma Membrane
As Well As Throughout The Cytoplasm During Metaphase
Subsequently Concentrated At Patches In The Equatorial Region At The Onset Of Cytokinesis
And Becomes Distributed In The Equatorial Region Concurrent With Cleavage Furrow Ingression
In Late Stages Of Cytokinesis
Concentrates At The Midbody Ring/Flemming Body
Recruitment To The Midbody Ring Requires Both Activation By Psd/Efa6a And Interaction With Kif23/Mklp1
After Abscission Of The Intercellular Bridge
Incorporated Into One Of The Daughter Cells As A Midbody Remnant And Localizes To Punctate Structures Beneath The Plasma Membrane
Recruited To The Cell Membrane In Association With Cyth2 And Arl4c
Colocalizes With Dab2ip At The Plasma Membrane And Endocytic Vesicles
Myristoylation Is Required For Proper Localization To Membranes: Myristoylation On Lys-3 Allows Arf6 To Remain On Membranes During The Gtpase Cycle
Alternative Antibody Names Anti-Adp-Ribosylation Factor 6 antibody
Anti-ARF6 antibody

Information sourced from Uniprot.org

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